Detailed information of TA system TA062416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2455471..2456249
• Replicon: chromosome
• Accession: NZ_CP015130
• Organism: Klebsiella pneumoniae strain Kpn555

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: WM92_RS12200
• Protein ID: WP_031591140.1
• Coordinates: 2455471..2455959 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: WM92_RS12205
• Protein ID: WP_004150912.1
• Coordinates: 2455956..2456249 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM92_RS12195	2451648..2455390	+	3743	Protein_2378	hypothetical protein	-
WM92_RS12200	2455471..2455959	-	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
WM92_RS12205	2455956..2456249	-	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
WM92_RS12210	2456893..2458269	+	1377	WP_004217775.1	cystathionine beta-synthase	-
WM92_RS12215	2458280..2459428	+	1149	WP_004181305.1	PLP-dependent aspartate aminotransferase family protein	-
WM92_RS12220	2459429..2460340	-	912	WP_064839591.1	LysR family transcriptional regulator	-
WM92_RS12225	2460438..2461040	+	603	WP_004174410.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T62416 WP_031591140.1 NZ_CP015130:c2455959-2455471 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T62416 NZ_CP015130:c2455959-2455471 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTGTGTTGCGGCAGCGATTCTAACGTAC
TGGCGTATTACTCGCTCGCGTCCAGTGCGGTAATGACTAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTAGTAGTGCTGGGGCGTCTGGCGGTGGATCAGTCTCTACATGGACGGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGGGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTATCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT62416 WP_004150912.1 NZ_CP015130:c2456249-2455956 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT62416 NZ_CP015130:c2456249-2455956 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4