Detailed information of TA system TA062405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 49189..49796
• Replicon: plasmid p3
• Accession: NZ_CP015123
• Organism: Enterococcus faecium strain E39

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: XM37_RS15165
• Protein ID: WP_002321032.1
• Coordinates: 49446..49796 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: XM37_RS15160
• Protein ID: WP_002287514.1
• Coordinates: 49189..49452 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XM37_RS15120	44539..45012	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
XM37_RS15125	45378..45638	+	261	WP_002321303.1	hypothetical protein	-
XM37_RS15130	46083..46289	+	207	WP_002296239.1	hypothetical protein	-
XM37_RS15135	46289..46540	+	252	WP_002305766.1	hypothetical protein	-
XM37_RS15140	46556..46972	+	417	WP_002305764.1	hypothetical protein	-
XM37_RS15145	46954..47226	+	273	WP_002348912.1	hypothetical protein	-
XM37_RS15150	47393..47794	+	402	WP_002305759.1	IS200/IS605 family transposase	-
XM37_RS15155	47806..48951	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
XM37_RS15160	49189..49452	+	264	WP_002287514.1	hypothetical protein	Antitoxin
XM37_RS15165	49446..49796	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XM37_RS15170	49820..50434	-	615	WP_002318230.1	recombinase family protein	-
XM37_RS15175	50748..51170	+	423	WP_002288787.1	hypothetical protein	-
XM37_RS15180	51180..51383	+	204	WP_002299573.1	hypothetical protein	-
XM37_RS15185	51594..52178	+	585	WP_002299575.1	recombinase family protein	-
XM37_RS15190	52347..53672	+	1326	WP_002303115.1	Y-family DNA polymerase	-
XM37_RS15195	53665..54015	+	351	WP_002303114.1	hypothetical protein	-
XM37_RS15200	54012..54191	+	180	WP_002322386.1	hypothetical protein	-
XM37_RS15205	54203..54496	+	294	WP_002322251.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56285	56285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T62405 WP_002321032.1 NZ_CP015123:49446-49796 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T62405 NZ_CP015123:49446-49796 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT62405 WP_002287514.1 NZ_CP015123:49189-49452 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT62405 NZ_CP015123:49189-49452 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0