Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1821267..1821857 Replicon chromosome
Accession NZ_CP015120
Organism Klebsiella pneumoniae strain kp757

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag A4U70_RS08995 Protein ID WP_022615588.1
Coordinates 1821525..1821857 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag A4U70_RS08990 Protein ID WP_000288812.1
Coordinates 1821267..1821524 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4U70_RS08965 1817602..1819350 + 1749 WP_023302666.1 hypothetical protein -
A4U70_RS08970 1819429..1819985 + 557 Protein_1730 hypothetical protein -
A4U70_RS08975 1820268..1820729 + 462 WP_004213450.1 hypothetical protein -
A4U70_RS08980 1820726..1820932 + 207 WP_022615589.1 helix-turn-helix domain-containing protein -
A4U70_RS08990 1821267..1821524 + 258 WP_000288812.1 antitoxin Antitoxin
A4U70_RS08995 1821525..1821857 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A4U70_RS09005 1822179..1823615 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
A4U70_RS09015 1823981..1825435 - 1455 WP_004148975.1 AMP nucleosidase -
A4U70_RS09020 1825565..1825810 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T62388 WP_022615588.1 NZ_CP015120:1821525-1821857 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T62388 NZ_CP015120:1821525-1821857 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT62388 WP_000288812.1 NZ_CP015120:1821267-1821524 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT62388 NZ_CP015120:1821267-1821524 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References