Detailed information of TA system TA062385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 6282322..6283364
• Replicon: chromosome
• Accession: NZ_CP015117
• Organism: Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: A4W92_RS29515
• Protein ID: WP_003153636.1
• Coordinates: 6282789..6283364 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: A4W92_RS29510
• Protein ID: WP_003050245.1
• Coordinates: 6282322..6282792 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4W92_RS29475	6277714..6279132	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
A4W92_RS29480	6279122..6280033	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
A4W92_RS29485	6280030..6280722	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
A4W92_RS29490	6280719..6281117	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
A4W92_RS29495	6281129..6281488	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
A4W92_RS29500	6281505..6281738	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
A4W92_RS29505	6281735..6282118	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
A4W92_RS29510	6282322..6282792	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
A4W92_RS29515	6282789..6283364	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
A4W92_RS29520	6283382..6284296	+	915	WP_016852809.1	AAA family ATPase	-
A4W92_RS29525	6284293..6284763	+	471	WP_003090160.1	hypothetical protein	-
A4W92_RS29530	6284760..6285260	+	501	WP_003090159.1	hypothetical protein	-
A4W92_RS29535	6285260..6286162	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
A4W92_RS29540	6286201..6286926	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	6207758..6329462	121704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T62385 WP_003153636.1 NZ_CP015117:6282789-6283364 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T62385 NZ_CP015117:6282789-6283364 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT62385 WP_003050245.1 NZ_CP015117:6282322-6282792 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT62385 NZ_CP015117:6282322-6282792 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68