Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 2292575..2293161 Replicon chromosome
Accession NZ_CP015117
Organism Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)


Gene name PumA Uniprot ID G8CP73
Locus tag A4W92_RS10700 Protein ID WP_003120987.1
Coordinates 2292862..2293161 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag A4W92_RS10695 Protein ID WP_003448662.1
Coordinates 2292575..2292865 (-) Length 97 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4W92_RS10675 2287726..2287935 + 210 WP_003105733.1 cold-shock protein -
A4W92_RS10680 2288157..2290046 + 1890 WP_016851610.1 hypothetical protein -
A4W92_RS10685 2290043..2292019 + 1977 WP_016851611.1 DEAD/DEAH box helicase -
A4W92_RS10690 2292160..2292504 + 345 WP_016851612.1 hypothetical protein -
A4W92_RS10695 2292575..2292865 - 291 WP_003448662.1 putative addiction module antidote protein Antitoxin
A4W92_RS10700 2292862..2293161 - 300 WP_003120987.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4W92_RS10705 2293363..2294487 + 1125 WP_012076859.1 TcpQ domain-containing protein -
A4W92_RS10710 2294487..2296196 + 1710 WP_012076860.1 PilN family type IVB pilus formation outer membrane protein -
A4W92_RS10715 2296200..2297525 + 1326 WP_003099758.1 type 4b pilus protein PilO2 -
A4W92_RS10720 2297515..2298048 + 534 WP_003099760.1 type IV pilus biogenesis protein PilP -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2266451..2368432 101981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 100 a.a.        Molecular weight: 11133.88 Da        Isoelectric Point: 10.4495

>T62381 WP_003120987.1 NZ_CP015117:c2293161-2292862 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Download         Length: 300 bp

>T62381 NZ_CP015117:c2293161-2292862 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10394.91 Da        Isoelectric Point: 4.7527

>AT62381 WP_003448662.1 NZ_CP015117:c2292865-2292575 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Download         Length: 291 bp

>AT62381 NZ_CP015117:c2292865-2292575 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G8CP73


Antitoxin

Source ID Structure

References