Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 1896554..1897162 Replicon chromosome
Accession NZ_CP015117
Organism Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)


Gene name PfiT Uniprot ID A0A1B1XUZ2
Locus tag A4W92_RS08865 Protein ID WP_003123043.1
Coordinates 1896554..1896901 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag A4W92_RS08870 Protein ID WP_003114155.1
Coordinates 1896911..1897162 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4W92_RS08845 1892830..1894062 + 1233 WP_016852057.1 phosphoadenosine phosphosulfate reductase family protein -
A4W92_RS33395 1894073..1894249 + 177 WP_016852058.1 hypothetical protein -
A4W92_RS08850 1894246..1894614 + 369 WP_016852059.1 ASCH domain-containing protein -
A4W92_RS32135 1894807..1895010 + 204 WP_003098423.1 AlpA family phage regulatory protein -
A4W92_RS08860 1895017..1896219 - 1203 WP_016852061.1 integrase family protein -
A4W92_RS08865 1896554..1896901 - 348 WP_003123043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4W92_RS08870 1896911..1897162 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A4W92_RS08875 1897376..1898359 - 984 WP_016852062.1 tyrosine-type recombinase/integrase -
A4W92_RS08880 1898359..1899651 - 1293 WP_003115206.1 hypothetical protein -
A4W92_RS08885 1899910..1901172 - 1263 WP_023093179.1 hypothetical protein -
A4W92_RS08890 1901174..1901524 - 351 WP_003159569.1 DUF2523 domain-containing protein -
A4W92_RS08895 1901534..1902136 - 603 WP_063174111.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1757251..1908482 151231


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12990.74 Da        Isoelectric Point: 4.4212

>T62380 WP_003123043.1 NZ_CP015117:c1896901-1896554 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T62380 NZ_CP015117:c1896901-1896554 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT62380 WP_003114155.1 NZ_CP015117:c1897162-1896911 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT62380 NZ_CP015117:c1897162-1896911 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1XUZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References