Detailed information of TA system TA062376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 209777..210387
• Replicon: plasmid unnamed
• Accession: NZ_CP015116
• Organism: Ralstonia solanacearum strain EP1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A454TTM1
• Locus tag: AC251_RS19590
• Protein ID: WP_058907722.1
• Coordinates: 210079..210387 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AC251_RS19585
• Protein ID: WP_016722339.1
• Coordinates: 209777..210082 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC251_RS19560	205275..205760	+	486	WP_016726511.1	hypothetical protein	-
AC251_RS19565	206091..206372	-	282	WP_058907724.1	hypothetical protein	-
AC251_RS19570	206504..206788	-	285	WP_011003391.1	hypothetical protein	-
AC251_RS27765	207093..207310	+	218	Protein_177	transposase	-
AC251_RS19575	207477..208145	+	669	WP_071507362.1	hypothetical protein	-
AC251_RS19580	208251..209564	-	1314	WP_071507363.1	metabolite/H+ symporter	-
AC251_RS19585	209777..210082	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
AC251_RS19590	210079..210387	-	309	WP_058907722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AC251_RS19595	210463..211662	-	1200	WP_058907721.1	MFS transporter	-
AC251_RS19600	211789..212610	-	822	WP_011003398.1	GntR family transcriptional regulator	-
AC251_RS19605	212788..213339	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
AC251_RS19610	213472..214071	+	600	WP_071507364.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / htpB / adeG / cheY / cheB / cheW / motA	1..2093441	2093441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11863.66 Da
• Isoelectric Point: 7.8930

>T62376 WP_058907722.1 NZ_CP015116:c210387-210079 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T62376 NZ_CP015116:c210387-210079 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCAGGCAACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT62376 WP_016722339.1 NZ_CP015116:c210082-209777 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT62376 NZ_CP015116:c210082-209777 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACAGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A454TTM1