Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2234851..2236437 Replicon chromosome
Accession NZ_CP015115
Organism Ralstonia solanacearum strain EP1

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag AC251_RS10425 Protein ID WP_071507716.1
Coordinates 2235100..2236437 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag AC251_RS10420 Protein ID WP_028853251.1
Coordinates 2234851..2235096 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC251_RS10390 2230914..2231840 - 927 WP_011001401.1 muramoyltetrapeptide carboxypeptidase -
AC251_RS10395 2231837..2232388 - 552 WP_016727233.1 tRNA adenosine(34) deaminase TadA -
AC251_RS10400 2232408..2232884 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
AC251_RS10405 2232952..2233590 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
AC251_RS10415 2233908..2234747 + 840 WP_011001397.1 oxaloacetate decarboxylase -
AC251_RS10420 2234851..2235096 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
AC251_RS10425 2235100..2236437 + 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
AC251_RS10430 2236434..2237636 + 1203 WP_071507715.1 MFS transporter -
AC251_RS10435 2237862..2238590 + 729 WP_011001393.1 DsbA family protein -
AC251_RS10440 2238613..2239176 - 564 WP_071654081.1 RNA 2',3'-cyclic phosphodiesterase -
AC251_RS10445 2239357..2240055 - 699 WP_071507713.1 protocatechuate 3,4-dioxygenase subunit alpha -
AC251_RS10450 2240052..2240792 - 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T62375 WP_071507716.1 NZ_CP015115:2235100-2236437 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T62375 NZ_CP015115:2235100-2236437 [Ralstonia solanacearum]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGCCTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACAGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGACCGCATCGACAGCGAGCCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCACGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCGCCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGCCGCTGGATGCCGGGCAACGCCTGGATCGCGCGCCTGCCGCAGGAAGACTTCTGCCAGGCCACCGGCACGCCCGC
GCACCGCAAATACGAAAACGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTGCTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTATGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGCTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCGAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT62375 WP_028853251.1 NZ_CP015115:2234851-2235096 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT62375 NZ_CP015115:2234851-2235096 [Ralstonia solanacearum]
ATGGAGTCCCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTGCTGCGCATCCTGCACGCGCTCGGCGCGCAGCTGACGCTGCGCACGTCCGCGCCCGCCGCCGCCGCGCCCGCCGAC
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References