Detailed information of TA system TA062372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2559063..2559577
• Replicon: chromosome
• Accession: NZ_CP015114
• Organism: Staphylococcus condimenti strain DSM 11674

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A4G25_RS12300
• Protein ID: WP_082107871.1
• Coordinates: 2559227..2559577 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B9DMI8
• Locus tag: A4G25_RS12295
• Protein ID: WP_015900820.1
• Coordinates: 2559063..2559230 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4G25_RS12270	2554984..2555454	+	471	WP_047132374.1	PH domain-containing protein	-
A4G25_RS12275	2555447..2556919	+	1473	WP_047132375.1	PH domain-containing protein	-
A4G25_RS12280	2556924..2557409	+	486	WP_063164645.1	PH domain-containing protein	-
A4G25_RS12285	2557411..2557764	+	354	WP_047132377.1	holo-ACP synthase	-
A4G25_RS12290	2557828..2558982	+	1155	WP_047132378.1	alanine racemase	-
A4G25_RS12295	2559063..2559230	+	168	WP_015900820.1	hypothetical protein	Antitoxin
A4G25_RS12300	2559227..2559577	+	351	WP_082107871.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A4G25_RS12305	2559845..2561161	-	1317	WP_063164607.1	ISL3 family transposase	-
A4G25_RS12310	2561406..2562407	+	1002	WP_047133046.1	PP2C family protein-serine/threonine phosphatase	-
A4G25_RS12315	2562484..2562810	+	327	WP_015900817.1	anti-sigma factor antagonist	-
A4G25_RS12320	2562812..2563291	+	480	WP_047133045.1	anti-sigma B factor RsbW	-
A4G25_RS12325	2563266..2564036	+	771	WP_047133044.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2559845..2561161	1316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13093.22 Da
• Isoelectric Point: 10.0708

>T62372 WP_082107871.1 NZ_CP015114:2559227-2559577 [Staphylococcus condimenti]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAMTGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Nucleotide

• Length: 351 bp

>T62372 NZ_CP015114:2559227-2559577 [Staphylococcus condimenti]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCGCCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAACGACACTGGAAATAAGTATAGTCCAACAGTCATTGTAGCTGCAATGACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCAGTTATTTTACTAGAACAGATAAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6211.75 Da
• Isoelectric Point: 3.6836

>AT62372 WP_015900820.1 NZ_CP015114:2559063-2559230 [Staphylococcus condimenti]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Nucleotide

• Length: 168 bp

>AT62372 NZ_CP015114:2559063-2559230 [Staphylococcus condimenti]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A380F5Y4