Detailed information of TA system TA062355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 452471..452925
• Replicon: chromosome
• Accession: NZ_CP015113
• Organism: Kosakonia radicincitans strain GXGL-4A

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: A3780_RS02100
• Protein ID: WP_007373008.1
• Coordinates: 452471..452605 (+)
• Length: 45 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: A3780_RS02105
• Protein ID: WP_035886668.1
• Coordinates: 452620..452925 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3780_RS02075	447981..448664	+	684	WP_007373003.1	hypothetical protein	-
A3780_RS02080	448657..449124	+	468	WP_007373004.1	YjiG family protein	-
A3780_RS02085	449149..450252	+	1104	WP_007373005.1	M20 peptidase aminoacylase family protein	-
A3780_RS02090	450254..450775	-	522	WP_035886585.1	VOC family protein	-
A3780_RS02095	450974..452344	+	1371	WP_035886583.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
A3780_RS02100	452471..452605	+	135	WP_007373008.1	BrnT family toxin	Toxin
A3780_RS02105	452620..452925	+	306	WP_035886668.1	BrnA antitoxin family protein	Antitoxin
A3780_RS02110	453003..453959	-	957	WP_007373010.1	GTPase	-
A3780_RS02115	453970..454173	-	204	WP_035886582.1	YbdD/YjiX family protein	-
A3780_RS02120	454328..456481	-	2154	WP_007373012.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-41)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 5328.82 Da
• Isoelectric Point: 4.6615

>T62355 WP_007373008.1 NZ_CP015113:452471-452605 [Kosakonia radicincitans]
MEFEWDVTKARSNQRKHGIRFEEAVSVFEDPYHLSIQDRFENGE

Nucleotide

• Length: 135 bp

>T62355 NZ_CP015113:452471-452605 [Kosakonia radicincitans]
ATGGAGTTTGAATGGGACGTCACTAAAGCAAGAAGTAATCAACGCAAGCATGGCATTCGCTTTGAAGAAGCCGTATCGGT
TTTCGAGGATCCTTACCATTTGTCCATCCAGGACCGGTTCGAGAATGGCGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11490.22 Da
• Isoelectric Point: 10.3140

>AT62355 WP_035886668.1 NZ_CP015113:452620-452925 [Kosakonia radicincitans]
MSMVKHKSGELPALNKQREAELKAQANKSDGEIDYSDIPSTDDVLWAGAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMMRDLQNKK

Nucleotide

• Length: 306 bp

>AT62355 NZ_CP015113:452620-452925 [Kosakonia radicincitans]
ATGAGCATGGTTAAACATAAATCGGGTGAGTTGCCAGCGCTCAACAAGCAGCGTGAAGCTGAATTAAAAGCGCAGGCAAA
TAAATCGGACGGAGAGATTGATTACAGCGATATTCCGTCCACAGATGACGTGTTATGGGCTGGCGCTGTGCGCGGTAAGT
TTTTTCGCCCGTTAAAAACGCAGGCATCGGTGCGTATTGATGCTGATGTGATGGAGTGGTTAAAACGACCGGGGAAAGGG
TATCAGACGCGGCTCAATGCCATTTTGCGTGAGGCAATGATGCGCGATTTGCAGAACAAGAAGTAA