Detailed information of TA system TA062353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4130..4715
• Replicon: plasmid unnamed2
• Accession: NZ_CP015112
• Organism: Acinetobacter sp. TGL-Y2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A143G982
• Locus tag: AMD27_RS17875
• Protein ID: WP_067664143.1
• Coordinates: 4130..4417 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A143G995
• Locus tag: AMD27_RS17880
• Protein ID: WP_067664146.1
• Coordinates: 4404..4715 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD27_RS17845	639..1241	+	603	WP_067664128.1	histidine phosphatase family protein	-
AMD27_RS17850	1251..1760	-	510	WP_067664131.1	hypothetical protein	-
AMD27_RS17855	1793..2041	-	249	WP_171254885.1	hypothetical protein	-
AMD27_RS17860	2204..2896	-	693	WP_067664137.1	immunity 52 family protein	-
AMD27_RS17865	2911..3543	-	633	WP_067664140.1	hypothetical protein	-
AMD27_RS17870	3607..3900	+	294	Protein_5	hypothetical protein	-
AMD27_RS17875	4130..4417	+	288	WP_067664143.1	BrnT family toxin	Toxin
AMD27_RS17880	4404..4715	+	312	WP_067664146.1	BrnA antitoxin family protein	Antitoxin
AMD27_RS17885	4784..5200	-	417	WP_067664149.1	carboxymuconolactone decarboxylase family protein	-
AMD27_RS17890	5310..5624	+	315	WP_067664152.1	helix-turn-helix transcriptional regulator	-
AMD27_RS17895	5820..6386	-	567	WP_067664155.1	plasmid replication DNA-binding protein	-
AMD27_RS17900	6450..7343	-	894	WP_067664158.1	replication initiation protein RepM	-
AMD27_RS17905	8264..8596	-	333	WP_067664160.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8682	8682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11110.43 Da
• Isoelectric Point: 4.6017

>T62353 WP_067664143.1 NZ_CP015112:4130-4417 [Acinetobacter sp. TGL-Y2]
MELYFEWDEAKNQKNQNKHDVSFETASLVFDDPLRISIQDRHTGGEERWQTIGMVKGVLMLLVAHTIFDEDDCEIIRIIS
AREVTKAERSQYEHG

Nucleotide

• Length: 288 bp

>T62353 NZ_CP015112:4130-4417 [Acinetobacter sp. TGL-Y2]
ATGGAACTGTATTTCGAATGGGATGAGGCAAAAAACCAAAAGAATCAGAATAAGCATGATGTTTCTTTTGAAACGGCAAG
CCTGGTTTTTGATGATCCTTTAAGGATATCAATCCAAGATCGACATACCGGTGGTGAAGAACGTTGGCAAACCATTGGTA
TGGTTAAAGGCGTACTGATGTTATTAGTAGCTCACACCATCTTCGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGGAAGTAACCAAAGCGGAGCGTAGTCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12072.77 Da
• Isoelectric Point: 9.4646

>AT62353 WP_067664146.1 NZ_CP015112:4404-4715 [Acinetobacter sp. TGL-Y2]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGPVPDEQLDLSDIPEITDWSNAVRHGQFYRPVKQQTSVRLDADVLAWLKDQ
GKGYQTRMNKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT62353 NZ_CP015112:4404-4715 [Acinetobacter sp. TGL-Y2]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGAAAACTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCTAAAGGTCCTGTACCAGATGAGCAATTAGACTTATCTGATATTCCCGAAATTACCGACTGGAGCAATGCTGTTCGCC
ATGGGCAATTCTATCGTCCAGTGAAGCAACAAACCTCTGTTCGCCTTGATGCTGATGTACTGGCATGGTTAAAAGATCAA
GGTAAGGGCTATCAGACACGTATGAATAAAATTTTGCGTGAAGCAATGTTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: A0A143G982

Antitoxin

• AlphaFold DB: A0A143G995