Detailed information of TA system TA062352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 198376..198936
• Replicon: plasmid unnamed1
• Accession: NZ_CP015111
• Organism: Acinetobacter sp. TGL-Y2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A143G8X3
• Locus tag: AMD27_RS17385
• Protein ID: WP_067663816.1
• Coordinates: 198547..198936 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A143G966
• Locus tag: AMD27_RS17380
• Protein ID: WP_067663813.1
• Coordinates: 198376..198546 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD27_RS17345	194080..194982	-	903	WP_067663796.1	hypothetical protein	-
AMD27_RS17350	195021..195293	-	273	WP_067663798.1	hypothetical protein	-
AMD27_RS17355	195320..195835	-	516	WP_067663801.1	hypothetical protein	-
AMD27_RS17360	195992..196381	+	390	WP_067663804.1	hypothetical protein	-
AMD27_RS17365	196687..196959	-	273	WP_067663807.1	hypothetical protein	-
AMD27_RS17370	197031..197534	-	504	WP_212846472.1	hypothetical protein	-
AMD27_RS17380	198376..198546	+	171	WP_067663813.1	acetyltransferase	Antitoxin
AMD27_RS17385	198547..198936	+	390	WP_067663816.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AMD27_RS17390	198950..199153	+	204	WP_067663818.1	hypothetical protein	-
AMD27_RS17395	199219..199557	-	339	WP_067663821.1	hypothetical protein	-
AMD27_RS17400	199849..200343	-	495	WP_067663826.1	hypothetical protein	-
AMD27_RS17405	200437..200769	-	333	WP_150115848.1	hypothetical protein	-
AMD27_RS17415	201631..201954	-	324	WP_067663836.1	toxin-antitoxin system HicB family antitoxin	-
AMD27_RS17420	201963..202208	-	246	WP_067663841.1	toxin HicA	-
AMD27_RS17425	202262..202690	-	429	WP_067663844.1	Arc family DNA-binding protein	-
AMD27_RS17430	202943..203482	-	540	WP_150115849.1	hypothetical protein	-
AMD27_RS17435	203496..203873	-	378	WP_067663849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..298877	298877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (30-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14136.02 Da
• Isoelectric Point: 4.1765

>T62352 WP_067663816.1 NZ_CP015111:198547-198936 [Acinetobacter sp. TGL-Y2]
MNLVQFPFERVIQINTIILSTGKGMKGTADLGKLQGALSRIDTAIHYQGLDDVFEIAAKYASSIATAHALSDANKRTGLA
VALEYLSLNDFELKLDEELLADAMRDLVIGEISESDFADILYSFYVSND

Nucleotide

• Length: 390 bp

>T62352 NZ_CP015111:198547-198936 [Acinetobacter sp. TGL-Y2]
ATGAATTTAGTACAGTTTCCTTTCGAGCGTGTAATTCAAATCAACACTATTATTCTTTCAACTGGTAAGGGGATGAAAGG
AACTGCGGATTTAGGAAAATTACAAGGTGCTTTATCTCGCATAGATACCGCCATTCATTATCAAGGATTGGATGACGTTT
TCGAAATAGCAGCGAAATATGCATCTAGTATTGCAACAGCTCATGCTTTATCTGATGCAAATAAACGTACAGGTTTAGCA
GTAGCGTTGGAATATTTGTCTTTAAACGATTTTGAATTAAAACTAGATGAAGAATTATTGGCAGATGCAATGAGAGATTT
GGTTATTGGTGAAATTTCGGAATCAGACTTTGCAGATATTCTATATTCTTTTTATGTTTCAAATGATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6491.63 Da
• Isoelectric Point: 10.6555

>AT62352 WP_067663813.1 NZ_CP015111:198376-198546 [Acinetobacter sp. TGL-Y2]
MENSNKKYGVTIVSRPKIKATKELNLSGKEGEQIVKSETKLVLMRHQKTFKRLEDM

Nucleotide

• Length: 171 bp

>AT62352 NZ_CP015111:198376-198546 [Acinetobacter sp. TGL-Y2]
ATGGAAAATTCAAATAAAAAATATGGTGTTACTATCGTTTCTCGCCCAAAGATCAAAGCAACCAAAGAGCTTAATCTAAG
CGGAAAAGAAGGTGAGCAAATTGTTAAATCTGAAACTAAATTAGTTTTAATGCGTCACCAAAAAACGTTCAAACGTTTGG
AAGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A143G8X3

Antitoxin

• AlphaFold DB: A0A143G966