Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-AbrB
Location 1143116..1143744 Replicon chromosome
Accession NZ_CP015103
Organism Thermococcus siculi strain RG-20

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag A3L11_RS06150 Protein ID WP_088856062.1
Coordinates 1143116..1143529 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag A3L11_RS06155 Protein ID WP_088856063.1
Coordinates 1143517..1143744 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3L11_RS06125 (A3L11_06125) 1139126..1139950 - 825 WP_088856057.1 type I-A CRISPR-associated protein Cas5a -
A3L11_RS06130 (A3L11_06130) 1139951..1140970 - 1020 WP_232461959.1 type I-A CRISPR-associated protein Cas7/Csa2 -
A3L11_RS06135 (A3L11_06135) 1140970..1141329 - 360 WP_088856059.1 hypothetical protein -
A3L11_RS06140 (A3L11_06140) 1141464..1142126 + 663 WP_088856060.1 BlaI/MecI/CopY family transcriptional regulator -
A3L11_RS06145 (A3L11_06145) 1142123..1143103 - 981 WP_088856061.1 type I-B CRISPR-associated endonuclease Cas1b -
A3L11_RS06150 (A3L11_06150) 1143116..1143529 - 414 WP_088856062.1 PIN domain-containing protein Toxin
A3L11_RS06155 (A3L11_06155) 1143517..1143744 - 228 WP_088856063.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A3L11_RS06160 (A3L11_06160) 1143801..1144280 - 480 WP_088856979.1 CRISPR-associated protein Cas4 -
A3L11_RS06165 (A3L11_06165) 1144376..1147195 - 2820 WP_088856064.1 CRISPR-associated helicase Cas3' -
A3L11_RS06170 (A3L11_06170) 1147195..1148046 - 852 WP_157727081.1 type I-B CRISPR-associated protein Cas5b -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-126)

Antitoxin

SpoVT-AbrB domain

(4-44)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16138.69 Da        Isoelectric Point: 8.3848

>T62346 WP_088856062.1 NZ_CP015103:c1143529-1143116 [Thermococcus siculi]
MHAVIDTNVLLYDTFEDLPFHHEARRLLDSLDRWYVPPIVLQEYVWFFKRKNLPVKLVRSMLSEYLDDPRFNRLNDNGES
IAYALELIEKNSLSLSRFNDAIILYHALQRGYPLATFDKKFRKLAVKNGVEVLPAKV

Download         Length: 414 bp

>T62346 NZ_CP015103:c1143529-1143116 [Thermococcus siculi]
ATGCATGCAGTGATAGATACTAACGTGCTCCTGTACGACACATTTGAAGATCTCCCATTTCATCACGAAGCCCGACGGCT
TTTGGATTCACTGGACCGCTGGTACGTACCCCCAATAGTTCTGCAGGAATACGTCTGGTTCTTCAAGAGGAAAAACCTGC
CCGTGAAGCTTGTAAGATCCATGCTGAGTGAGTATTTAGATGATCCTCGCTTTAACAGGCTGAACGATAACGGAGAGTCT
ATAGCCTACGCCCTTGAGCTTATAGAAAAGAACTCCTTGTCACTATCTCGTTTCAACGATGCTATAATCCTGTATCACGC
CCTCCAAAGGGGCTATCCCCTTGCCACGTTTGACAAAAAATTCAGAAAACTGGCAGTTAAAAATGGCGTGGAGGTTCTCC
CAGCGAAAGTATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8736.35 Da        Isoelectric Point: 7.9755

>AT62346 WP_088856063.1 NZ_CP015103:c1143744-1143517 [Thermococcus siculi]
MPVTKVTRNYQITLPAEIRKALGIKEGELLEVELEGEKIIVKRLKRKRKTLKLGERLTPEEIEKTIEEGMEECMQ

Download         Length: 228 bp

>AT62346 NZ_CP015103:c1143744-1143517 [Thermococcus siculi]
ATGCCTGTGACAAAGGTAACCCGGAACTATCAGATAACGCTCCCCGCGGAGATCAGAAAAGCCCTCGGGATTAAGGAGGG
GGAGCTGCTTGAGGTTGAGCTTGAAGGGGAAAAAATCATAGTCAAGAGGCTGAAGAGAAAGAGGAAAACCCTCAAACTAG
GTGAGAGGCTCACGCCTGAGGAGATTGAGAAGACCATTGAGGAGGGTATGGAAGAATGCATGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References