Detailed information of TA system TA062332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 44284..45320
• Replicon: plasmid pPABY7
• Accession: NZ_CP015097
• Organism: Pelagibaca abyssi strain JLT2014

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: Ga0080574_RS25515
• Protein ID: WP_076706364.1
• Coordinates: 44284..44859 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: Ga0080574_RS25520
• Protein ID: WP_076706365.1
• Coordinates: 44856..45320 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ga0080574_RS25500	40001..41542	+	1542	WP_076706361.1	succinate CoA transferase	-
Ga0080574_RS25505	41673..42926	-	1254	WP_076706362.1	saccharopine dehydrogenase family protein	-
Ga0080574_RS25510	43001..44092	-	1092	WP_076706363.1	carboxynorspermidine decarboxylase	-
Ga0080574_RS25515	44284..44859	-	576	WP_076706364.1	PIN domain-containing protein	Toxin
Ga0080574_RS25520	44856..45320	-	465	WP_076706365.1	helix-turn-helix domain-containing protein	Antitoxin
Ga0080574_RS25525	45764..46801	-	1038	WP_076706366.1	ParB N-terminal domain-containing protein	-
Ga0080574_RS25530	46798..48108	-	1311	WP_156876489.1	AAA family ATPase	-
Ga0080574_RS25535	48479..49177	+	699	WP_076706367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56541	56541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (11-115)

Antitoxin

• SinI-like, DNA-binding domain (85-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 20947.82 Da
• Isoelectric Point: 4.6364

>T62332 WP_076706364.1 NZ_CP015097:c44859-44284 [Pelagibaca abyssi]
MSRYADRFTALLDACVLGGALRRNMLLSLAEAGLFRPRWSNRVLDETQRAIARITKGDTDGSRQRAAINAAFPEAMVTGY
EIFEDRLALPDPDDNHVLAAAIATSASVIVTDNLADFPPEALAPHPIQAISADDFIADIIELDPSEAILALRRMRERFRN
PALNVAALIHKSEAQGLLQVATFMDEYQSSL

Nucleotide

• Length: 576 bp

>T62332 NZ_CP015097:c44859-44284 [Pelagibaca abyssi]
ATGAGCCGATACGCGGATCGGTTCACGGCGCTGCTGGATGCCTGCGTCCTGGGCGGCGCGCTGCGGCGGAACATGCTGCT
CAGCCTGGCCGAGGCCGGATTGTTCCGCCCCCGGTGGAGCAATCGCGTTCTCGACGAGACCCAGAGGGCGATTGCCCGGA
TCACCAAGGGGGACACCGATGGTTCCCGGCAACGCGCGGCCATCAACGCGGCGTTTCCCGAAGCCATGGTGACCGGCTAC
GAGATCTTTGAAGACAGGCTTGCCTTGCCCGATCCGGACGACAACCACGTTCTGGCGGCGGCCATTGCGACCTCGGCGTC
CGTCATCGTCACGGACAATCTCGCGGATTTCCCGCCCGAGGCTCTGGCCCCGCATCCGATCCAGGCGATTTCGGCAGACG
ACTTCATCGCCGATATTATCGAACTGGATCCGTCAGAGGCCATCCTCGCCCTGCGCCGCATGCGGGAGCGGTTCAGGAAC
CCCGCCCTCAATGTCGCGGCGCTGATTCACAAATCCGAGGCGCAGGGCCTCCTTCAGGTTGCGACCTTCATGGATGAGTA
TCAGTCTTCGTTGTAG

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17038.43 Da
• Isoelectric Point: 4.6755

>AT62332 WP_076706365.1 NZ_CP015097:c45320-44856 [Pelagibaca abyssi]
MDALRKEAFFDRLPDEVEIRNAEQLRTIVASQIKEGEPTTLSLALEGGEVRTVTLAPALTASLLEVLRLVSSGRGFRMIP
VEAELTTQQAADLLNVSRPFLVKLLEDGEIPFTKTGRHRRVRADDLFAYKEKRDASRSEALSDLAAMDAEDGLV

Nucleotide

• Length: 465 bp

>AT62332 NZ_CP015097:c45320-44856 [Pelagibaca abyssi]
ATGGACGCCCTCAGAAAAGAGGCATTCTTTGATCGTTTGCCCGACGAGGTCGAGATCAGGAACGCCGAACAGCTCAGAAC
GATTGTCGCCTCCCAGATCAAGGAGGGCGAGCCGACGACGTTGTCGCTTGCGCTGGAGGGCGGCGAAGTGCGCACCGTGA
CCCTTGCGCCCGCGCTGACAGCCTCGCTTCTTGAAGTGCTGCGGCTGGTCTCCAGCGGTCGCGGGTTCCGGATGATCCCC
GTCGAGGCGGAGTTGACGACGCAGCAGGCGGCAGACCTTCTCAATGTGTCCCGTCCGTTCCTGGTCAAGCTTCTCGAAGA
CGGTGAAATCCCGTTCACGAAGACCGGCCGCCATCGTCGTGTTCGCGCGGATGATCTCTTTGCCTACAAGGAAAAGCGCG
ACGCGTCTCGTTCCGAGGCCCTGAGCGATCTTGCCGCCATGGATGCCGAAGACGGTCTTGTATGA