Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42287..42812 Replicon plasmid unnamed
Accession NZ_CP015086
Organism Escherichia coli O25b:H4

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag WLH_RS28060 Protein ID WP_001159868.1
Coordinates 42507..42812 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag WLH_RS28055 Protein ID WP_000813634.1
Coordinates 42287..42505 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WLH_RS28035 37398..37748 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
WLH_RS28040 37745..38170 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
WLH_RS28045 38392..40617 - 2226 WP_000698737.1 phage T7 exclusion protein -
WLH_RS28050 40619..41707 - 1089 WP_000952217.1 transcriptional repressor PifC -
WLH_RS28055 42287..42505 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
WLH_RS28060 42507..42812 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
WLH_RS28065 42813..43619 + 807 WP_000016982.1 site-specific integrase -
WLH_RS28070 44393..45148 + 756 WP_000852146.1 replication initiation protein RepE -
WLH_RS28075 45736..46902 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 - 1..130920 130920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T62318 WP_001159868.1 NZ_CP015086:42507-42812 [Escherichia coli O25b:H4]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T62318 NZ_CP015086:42507-42812 [Escherichia coli O25b:H4]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT62318 WP_000813634.1 NZ_CP015086:42287-42505 [Escherichia coli O25b:H4]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT62318 NZ_CP015086:42287-42505 [Escherichia coli O25b:H4]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References