Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4270392..4271010 Replicon chromosome
Accession NZ_CP015085
Organism Escherichia coli O25b:H4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag WLH_RS22125 Protein ID WP_001291435.1
Coordinates 4270392..4270610 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag WLH_RS22130 Protein ID WP_000344800.1
Coordinates 4270636..4271010 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WLH_RS22090 4265679..4266251 + 573 WP_000779831.1 YbaY family lipoprotein -
WLH_RS22095 4266282..4266593 - 312 WP_000409908.1 MGMT family protein -
WLH_RS22105 4266972..4267325 + 354 WP_000878135.1 DUF1428 family protein -
WLH_RS22110 4267367..4268917 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
WLH_RS22115 4269081..4269551 - 471 WP_000136192.1 YlaC family protein -
WLH_RS22120 4269667..4270218 - 552 WP_000102539.1 maltose O-acetyltransferase -
WLH_RS22125 4270392..4270610 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
WLH_RS22130 4270636..4271010 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
WLH_RS22135 4271556..4274705 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
WLH_RS22140 4274728..4275921 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62312 WP_001291435.1 NZ_CP015085:c4270610-4270392 [Escherichia coli O25b:H4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62312 NZ_CP015085:c4270610-4270392 [Escherichia coli O25b:H4]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT62312 WP_000344800.1 NZ_CP015085:c4271010-4270636 [Escherichia coli O25b:H4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT62312 NZ_CP015085:c4271010-4270636 [Escherichia coli O25b:H4]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References