62309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3709534..3710110 Replicon chromosome
Accession NZ_CP015085
Organism Escherichia coli O25b:H4

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag WLH_RS19315 Protein ID WP_001295743.1
Coordinates 3709534..3709821 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag WLH_RS19320 Protein ID WP_000063148.1
Coordinates 3709808..3710110 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WLH_RS19300 3705194..3706498 - 1305 WP_000535012.1 restriction endonuclease subunit S -
WLH_RS19305 3706488..3708107 - 1620 WP_001029745.1 type I restriction-modification system subunit M -
WLH_RS19310 3708171..3709337 - 1167 WP_000800831.1 restriction endonuclease -
WLH_RS19315 3709534..3709821 + 288 WP_001295743.1 BrnT family toxin Toxin
WLH_RS19320 3709808..3710110 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
WLH_RS19325 3710144..3711100 - 957 WP_001295745.1 GTPase -
WLH_RS19330 3711111..3711314 - 204 WP_000467859.1 YbdD/YjiX family protein -
WLH_RS19335 3711364..3713514 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T62309 WP_001295743.1 NZ_CP015085:3709534-3709821 [Escherichia coli O25b:H4]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T62309 NZ_CP015085:3709534-3709821 [Escherichia coli O25b:H4]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT62309 WP_000063148.1 NZ_CP015085:3709808-3710110 [Escherichia coli O25b:H4]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT62309 NZ_CP015085:3709808-3710110 [Escherichia coli O25b:H4]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References