Detailed information of TA system TA062308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3659853..3660673
• Replicon: chromosome
• Accession: NZ_CP015085
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: WLH_RS19045
• Protein ID: WP_001054379.1
• Coordinates: 3660416..3660673 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: WLH_RS19040
• Protein ID: WP_000123957.1
• Coordinates: 3659853..3660404 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS19015	3655547..3656860	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
WLH_RS19020	3656872..3657150	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
WLH_RS19025	3657147..3658268	-	1122	WP_000010833.1	M42 family metallopeptidase	-
WLH_RS29505	3658513..3658629	-	117	Protein_3466	VOC family protein	-
WLH_RS19030	3658667..3658927	-	261	WP_000077645.1	hypothetical protein	-
WLH_RS19035	3659055..3659801	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
WLH_RS19040	3659853..3660404	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
WLH_RS19045	3660416..3660673	-	258	WP_001054379.1	YjhX family toxin	Toxin
WLH_RS19055	3661050..3661214	-	165	Protein_3471	GNAT family N-acetyltransferase	-
WLH_RS19065	3662457..3663563	+	1107	Protein_3473	helicase YjhR	-
WLH_RS19070	3664146..3665126	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T62308 WP_001054379.1 NZ_CP015085:c3660673-3660416 [Escherichia coli O25b:H4]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T62308 NZ_CP015085:c3660673-3660416 [Escherichia coli O25b:H4]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT62308 WP_000123957.1 NZ_CP015085:c3660404-3659853 [Escherichia coli O25b:H4]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT62308 NZ_CP015085:c3660404-3659853 [Escherichia coli O25b:H4]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030