Detailed information of TA system TA062307

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3628469..3629304
• Replicon: chromosome
• Accession: NZ_CP015085
• Organism: Escherichia coli O25b:H4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: WLH_RS18885
• Protein ID: WP_000854759.1
• Coordinates: 3628927..3629304 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: WLH_RS18880
• Protein ID: WP_001295723.1
• Coordinates: 3628469..3628837 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WLH_RS18835	3623718..3624464	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
WLH_RS18840	3624545..3625513	+	969	Protein_3432	autotransporter domain-containing protein	-
WLH_RS18845	3625584..3625742	+	159	WP_001323397.1	DUF905 family protein	-
WLH_RS18855	3625897..3626715	+	819	WP_001234738.1	DUF945 domain-containing protein	-
WLH_RS18865	3627057..3627530	+	474	WP_001350782.1	antirestriction protein	-
WLH_RS18870	3627546..3628022	+	477	WP_001186775.1	RadC family protein	-
WLH_RS18875	3628085..3628306	+	222	WP_000692345.1	DUF987 domain-containing protein	-
WLH_RS18880	3628469..3628837	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WLH_RS18885	3628927..3629304	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
WLH_RS18890	3629301..3629789	+	489	WP_000761690.1	hypothetical protein	-
WLH_RS18895	3629806..3629982	+	177	WP_000839286.1	DUF957 domain-containing protein	-
WLH_RS29210	3630082..3630240	+	159	WP_001467148.1	hypothetical protein	-
WLH_RS18905	3630604..3630780	+	177	Protein_3443	helix-turn-helix domain-containing protein	-
WLH_RS18910	3631553..3633193	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T62307 WP_000854759.1 NZ_CP015085:3628927-3629304 [Escherichia coli O25b:H4]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T62307 NZ_CP015085:3628927-3629304 [Escherichia coli O25b:H4]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT62307 WP_001295723.1 NZ_CP015085:3628469-3628837 [Escherichia coli O25b:H4]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT62307 NZ_CP015085:3628469-3628837 [Escherichia coli O25b:H4]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52