Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1814397..1815051 Replicon chromosome
Accession NZ_CP015085
Organism Escherichia coli O25b:H4

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag WLH_RS09390 Protein ID WP_000244765.1
Coordinates 1814397..1814804 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag WLH_RS09395 Protein ID WP_000354050.1
Coordinates 1814785..1815051 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WLH_RS09370 1810354..1812087 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
WLH_RS09375 1812093..1812803 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
WLH_RS09380 1812828..1813724 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
WLH_RS09385 1813836..1814357 + 522 WP_001055874.1 flavodoxin FldB -
WLH_RS09390 1814397..1814804 - 408 WP_000244765.1 protein YgfX Toxin
WLH_RS09395 1814785..1815051 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
WLH_RS09400 1815294..1816274 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
WLH_RS09405 1816351..1817010 - 660 WP_000250275.1 hemolysin III family protein -
WLH_RS09410 1817174..1817485 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
WLH_RS09415 1817530..1818963 + 1434 WP_048266439.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T62297 WP_000244765.1 NZ_CP015085:c1814804-1814397 [Escherichia coli O25b:H4]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T62297 NZ_CP015085:c1814804-1814397 [Escherichia coli O25b:H4]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT62297 WP_000354050.1 NZ_CP015085:c1815051-1814785 [Escherichia coli O25b:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT62297 NZ_CP015085:c1815051-1814785 [Escherichia coli O25b:H4]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References