Detailed information of TA system TA062285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2307297..2307886
• Replicon: chromosome
• Accession: NZ_CP015081
• Organism: Deinococcus radiodurans R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX98
• Locus tag: A2G07_RS11445
• Protein ID: WP_010887062.1
• Coordinates: 2307297..2307650 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q9RX99
• Locus tag: A2G07_RS11450
• Protein ID: WP_010887061.1
• Coordinates: 2307644..2307886 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2G07_RS11415	2302508..2302687	+	180	WP_028328008.1	hypothetical protein	-
A2G07_RS11420	2302808..2303584	+	777	WP_010887067.1	trans-aconitate 2-methyltransferase	-
A2G07_RS11425	2303740..2303964	+	225	WP_162177572.1	hypothetical protein	-
A2G07_RS11430	2304145..2306613	-	2469	WP_010887065.1	ATP-dependent helicase HrpB	-
A2G07_RS11435	2306610..2307023	-	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	-
A2G07_RS11440	2307020..2307244	-	225	WP_162177573.1	hypothetical protein	-
A2G07_RS11445	2307297..2307650	-	354	WP_010887062.1	endoribonuclease MazF	Toxin
A2G07_RS11450	2307644..2307886	-	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A2G07_RS11455	2308066..2309547	+	1482	Protein_2261	carboxylesterase/lipase family protein	-
A2G07_RS11460	2309595..2310224	-	630	WP_027479568.1	HAD family hydrolase	-
A2G07_RS11465	2310226..2310405	-	180	WP_034349506.1	hypothetical protein	-
A2G07_RS11470	2310428..2311390	-	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
A2G07_RS11475	2311411..2312094	-	684	WP_010887056.1	SDR family oxidoreductase	-
A2G07_RS11480	2312091..2312462	-	372	Protein_2266	inorganic pyrophosphatase	-
A2G07_RS11485	2312455..2312874	-	420	WP_010887054.1	DinB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12450.24 Da
• Isoelectric Point: 7.3219

>T62285 WP_010887062.1 NZ_CP015081:c2307650-2307297 [Deinococcus radiodurans R1]
MVSDYVPDAGHLVWLNFTPQAGHEQGGRRPALVLSPAAYNGVTGLMQACPVTSRAKGYPFEVTLPAHLGVSGVVLADHCR
SLDWRSRRAEQLAEAPADVLAEVRGKLGSLLGMSEKA

Nucleotide

• Length: 354 bp

>T62285 NZ_CP015081:c2307650-2307297 [Deinococcus radiodurans R1]
ATGGTAAGCGATTATGTCCCGGACGCTGGGCATCTGGTGTGGCTCAACTTCACACCTCAGGCAGGGCATGAACAGGGGGG
ACGGCGACCGGCGCTGGTGCTTTCGCCTGCCGCTTATAACGGCGTCACTGGACTGATGCAGGCGTGTCCGGTGACCAGCC
GCGCCAAGGGGTATCCGTTTGAAGTGACGCTCCCTGCTCACCTTGGAGTCAGCGGCGTGGTGCTGGCCGACCATTGCCGC
AGCCTCGACTGGCGCAGTCGCCGGGCCGAACAACTTGCGGAGGCGCCGGCGGACGTCCTGGCCGAAGTCCGTGGCAAGTT
GGGCAGCTTGCTGGGCATGTCCGAGAAGGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8911.12 Da
• Isoelectric Point: 4.3178

>AT62285 WP_010887061.1 NZ_CP015081:c2307886-2307644 [Deinococcus radiodurans R1]
MTSQIQKWGNSLALRIPKALAQQVGLTQSSEVELLLQDGQIVIRPVPARQYDLAALLAEMTPENLHGETDWGALEGREEW

Nucleotide

• Length: 243 bp

>AT62285 NZ_CP015081:c2307886-2307644 [Deinococcus radiodurans R1]
ATGACGAGTCAAATTCAGAAATGGGGCAACAGCCTCGCGCTCCGCATTCCCAAAGCTCTGGCGCAGCAGGTGGGACTGAC
GCAGAGTTCAGAAGTGGAGCTGCTTCTTCAGGACGGTCAGATTGTCATCCGGCCAGTTCCTGCTCGGCAGTACGATCTCG
CCGCGCTGCTGGCCGAAATGACACCTGAAAATCTGCATGGGGAAACAGACTGGGGCGCACTGGAAGGACGCGAGGAATGG
TAA

Structures

Toxin

• PDB: 7D2M
• PDB: 7D2P
• PDB: 7DHP
• PDB: 7D2Q
• PDB: 7D28
• PDB: 7D2N

Antitoxin

• PDB: 7D2P
• PDB: 7D2Q
• PDB: 7D2N
• AlphaFold DB: Q9RX99