Detailed information of TA system TA062283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/VapC(toxin)
• Location: 2306610..2307244
• Replicon: chromosome
• Accession: NZ_CP015081
• Organism: Deinococcus radiodurans R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX96
• Locus tag: A2G07_RS11435
• Protein ID: WP_010887064.1
• Coordinates: 2306610..2307023 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A2G07_RS11440
• Protein ID: WP_162177573.1
• Coordinates: 2307020..2307244 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2G07_RS11410	2301885..2302511	+	627	WP_010887068.1	single-stranded DNA-binding protein DdrA	-
A2G07_RS11415	2302508..2302687	+	180	WP_028328008.1	hypothetical protein	-
A2G07_RS11420	2302808..2303584	+	777	WP_010887067.1	trans-aconitate 2-methyltransferase	-
A2G07_RS11425	2303740..2303964	+	225	WP_162177572.1	hypothetical protein	-
A2G07_RS11430	2304145..2306613	-	2469	WP_010887065.1	ATP-dependent helicase HrpB	-
A2G07_RS11435	2306610..2307023	-	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	Toxin
A2G07_RS11440	2307020..2307244	-	225	WP_162177573.1	hypothetical protein	Antitoxin
A2G07_RS11445	2307297..2307650	-	354	WP_010887062.1	endoribonuclease MazF	-
A2G07_RS11450	2307644..2307886	-	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
A2G07_RS11455	2308066..2309547	+	1482	Protein_2261	carboxylesterase/lipase family protein	-
A2G07_RS11460	2309595..2310224	-	630	WP_027479568.1	HAD family hydrolase	-
A2G07_RS11465	2310226..2310405	-	180	WP_034349506.1	hypothetical protein	-
A2G07_RS11470	2310428..2311390	-	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
A2G07_RS11475	2311411..2312094	-	684	WP_010887056.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14634.68 Da
• Isoelectric Point: 5.5820

>T62283 WP_010887064.1 NZ_CP015081:c2307023-2306610 [Deinococcus radiodurans R1]
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP

Nucleotide

• Length: 414 bp

>T62283 NZ_CP015081:c2307023-2306610 [Deinococcus radiodurans R1]
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8229.23 Da
• Isoelectric Point: 4.3481

>AT62283 WP_162177573.1 NZ_CP015081:c2307244-2307020 [Deinococcus radiodurans R1]
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE

Nucleotide

• Length: 225 bp

>AT62283 NZ_CP015081:c2307244-2307020 [Deinococcus radiodurans R1]
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA

Structures

Toxin

• AlphaFold DB: Q9RX96