Detailed information of TA system TA062271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 75498..76141
• Replicon: plasmid pEC448_1
• Accession: NZ_CP015077
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: A4R37_RS25375
• Protein ID: WP_001034044.1
• Coordinates: 75498..75914 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: A4R37_RS25380
• Protein ID: WP_001261286.1
• Coordinates: 75911..76141 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS25350	70644..71501	+	858	WP_000968139.1	iron/manganese ABC transporter permease subunit SitD	-
A4R37_RS25355	71900..72597	+	698	Protein_90	IS1 family transposase	-
A4R37_RS25365	72851..73873	-	1023	WP_000361402.1	helicase UvrD	-
A4R37_RS25370	73858..75423	-	1566	WP_001128474.1	AAA family ATPase	-
A4R37_RS25375	75498..75914	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
A4R37_RS25380	75911..76141	-	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A4R37_RS25385	76522..76911	+	390	Protein_95	pcar	-
A4R37_RS25390	76976..77215	+	240	Protein_96	IS6 family transposase	-
A4R37_RS25395	77267..77971	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
A4R37_RS25400	78035..78373	+	339	Protein_98	recombinase family protein	-
A4R37_RS25405	78556..79416	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
A4R37_RS28010	79563..79844	+	282	Protein_100	transposase zinc-binding domain-containing protein	-
A4R37_RS25410	80014..80718	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..133735	133735
	-	inside	IScluster/Tn	sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	65816..85913	20097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T62271 WP_001034044.1 NZ_CP015077:c75914-75498 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T62271 NZ_CP015077:c75914-75498 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT62271 WP_001261286.1 NZ_CP015077:c76141-75911 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT62271 NZ_CP015077:c76141-75911 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6