Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 35454..36013 Replicon plasmid pEC448_1
Accession NZ_CP015077
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag A4R37_RS25130 Protein ID WP_000421272.1
Coordinates 35454..35729 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag A4R37_RS25135 Protein ID WP_001387467.1
Coordinates 35729..36013 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS25105 30617..30847 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
A4R37_RS25110 30844..31260 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
A4R37_RS25115 31305..34349 - 3045 Protein_42 pcar -
A4R37_RS25120 34365..35062 - 698 Protein_43 IS1 family transposase -
A4R37_RS25125 35091..35348 - 258 WP_061092170.1 hypothetical protein -
A4R37_RS25130 35454..35729 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4R37_RS25135 35729..36013 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
A4R37_RS25140 36618..37370 + 753 WP_000562172.1 IclR family transcriptional regulator -
A4R37_RS25145 37416..38381 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
A4R37_RS25150 38414..38794 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
A4R37_RS25155 38819..39709 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
A4R37_RS25160 39942..40136 - 195 WP_000796505.1 hypothetical protein -
A4R37_RS25165 40110..40550 + 441 Protein_52 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 1..133735 133735
- flank IS/Tn - - 34365..34868 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T62270 WP_000421272.1 NZ_CP015077:c35729-35454 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T62270 NZ_CP015077:c35729-35454 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT62270 WP_001387467.1 NZ_CP015077:c36013-35729 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT62270 NZ_CP015077:c36013-35729 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References