62269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30617..31260 Replicon plasmid pEC448_1
Accession NZ_CP015077
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag A4R37_RS25110 Protein ID WP_001034046.1
Coordinates 30844..31260 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag A4R37_RS25105 Protein ID WP_001261278.1
Coordinates 30617..30847 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS25075 26128..26433 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
A4R37_RS25080 26435..26653 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
A4R37_RS25085 27220..27732 + 513 WP_000151784.1 hypothetical protein -
A4R37_RS25090 27766..28899 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
A4R37_RS25095 29066..29839 - 774 WP_000905949.1 hypothetical protein -
A4R37_RS25100 29852..30352 - 501 WP_000528931.1 hypothetical protein -
A4R37_RS25105 30617..30847 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A4R37_RS25110 30844..31260 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
A4R37_RS25115 31305..34349 - 3045 Protein_42 pcar -
A4R37_RS25120 34365..35062 - 698 Protein_43 IS1 family transposase -
A4R37_RS25125 35091..35348 - 258 WP_061092170.1 hypothetical protein -
A4R37_RS25130 35454..35729 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin -
A4R37_RS25135 35729..36013 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 1..133735 133735
- flank IS/Tn - - 34365..34868 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T62269 WP_001034046.1 NZ_CP015077:30844-31260 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T62269 NZ_CP015077:30844-31260 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT62269 WP_001261278.1 NZ_CP015077:30617-30847 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT62269 NZ_CP015077:30617-30847 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References