Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26128..26653 Replicon plasmid pEC448_1
Accession NZ_CP015077
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A4R37_RS25075 Protein ID WP_001159868.1
Coordinates 26128..26433 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A4R37_RS25080 Protein ID WP_000813634.1
Coordinates 26435..26653 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS25060 22038..23204 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
A4R37_RS25065 23792..24547 - 756 WP_000852146.1 replication initiation protein RepE -
A4R37_RS25070 25321..26127 - 807 WP_000016982.1 site-specific integrase -
A4R37_RS25075 26128..26433 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A4R37_RS25080 26435..26653 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A4R37_RS25085 27220..27732 + 513 WP_000151784.1 hypothetical protein -
A4R37_RS25090 27766..28899 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
A4R37_RS25095 29066..29839 - 774 WP_000905949.1 hypothetical protein -
A4R37_RS25100 29852..30352 - 501 WP_000528931.1 hypothetical protein -
A4R37_RS25105 30617..30847 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
A4R37_RS25110 30844..31260 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 1..133735 133735


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T62268 WP_001159868.1 NZ_CP015077:c26433-26128 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T62268 NZ_CP015077:c26433-26128 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT62268 WP_000813634.1 NZ_CP015077:c26653-26435 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT62268 NZ_CP015077:c26653-26435 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References