Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4990479..4991097 Replicon chromosome
Accession NZ_CP015076
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A4R37_RS24825 Protein ID WP_001291435.1
Coordinates 4990479..4990697 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A4R37_RS24830 Protein ID WP_000344800.1
Coordinates 4990723..4991097 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS24790 4985767..4986339 + 573 WP_000779841.1 YbaY family lipoprotein -
A4R37_RS24795 4986370..4986681 - 312 WP_000409908.1 MGMT family protein -
A4R37_RS24805 4987060..4987413 + 354 WP_000878147.1 DUF1428 family protein -
A4R37_RS24810 4987455..4989005 - 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A4R37_RS24815 4989169..4989639 - 471 WP_000136192.1 YlaC family protein -
A4R37_RS24820 4989755..4990306 - 552 WP_000102539.1 maltose O-acetyltransferase -
A4R37_RS24825 4990479..4990697 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A4R37_RS24830 4990723..4991097 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A4R37_RS24835 4991643..4994792 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
A4R37_RS24840 4994815..4996008 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62263 WP_001291435.1 NZ_CP015076:c4990697-4990479 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62263 NZ_CP015076:c4990697-4990479 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT62263 WP_000344800.1 NZ_CP015076:c4991097-4990723 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT62263 NZ_CP015076:c4991097-4990723 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References