Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4577729..4578279 Replicon chromosome
Accession NZ_CP015076
Organism Escherichia coli strain Ecol_448

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag A4R37_RS22910 Protein ID WP_001160966.1
Coordinates 4577965..4578279 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag A4R37_RS22905 Protein ID WP_000125566.1
Coordinates 4577729..4577962 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R37_RS22880 4572802..4573089 + 288 WP_000203741.1 ferredoxin-like protein FixX -
A4R37_RS22885 4573148..4574479 + 1332 WP_001183207.1 MFS transporter -
A4R37_RS22890 4574587..4575117 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
A4R37_RS22895 4575110..4576972 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
A4R37_RS22900 4577164..4577643 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
A4R37_RS22905 4577729..4577962 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
A4R37_RS22910 4577965..4578279 + 315 WP_001160966.1 CcdB family protein Toxin
A4R37_RS22915 4578276..4579124 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
A4R37_RS22920 4579131..4579508 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
A4R37_RS22925 4579511..4580332 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
A4R37_RS22930 4580329..4581318 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
A4R37_RS22935 4581318..4582604 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T62261 WP_001160966.1 NZ_CP015076:4577965-4578279 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T62261 NZ_CP015076:4577965-4578279 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT62261 WP_000125566.1 NZ_CP015076:4577729-4577962 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT62261 NZ_CP015076:4577729-4577962 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References