Detailed information of TA system TA062259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4428265..4429085
• Replicon: chromosome
• Accession: NZ_CP015076
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: A4R37_RS22110
• Protein ID: WP_001054379.1
• Coordinates: 4428828..4429085 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: A4R37_RS22105
• Protein ID: WP_000123957.1
• Coordinates: 4428265..4428816 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS22080	4423959..4425272	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
A4R37_RS22085	4425284..4425562	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A4R37_RS22090	4425559..4426680	-	1122	WP_000010833.1	M42 family metallopeptidase	-
A4R37_RS29765	4426925..4427041	-	117	Protein_4185	VOC family protein	-
A4R37_RS22095	4427079..4427339	-	261	WP_000077645.1	hypothetical protein	-
A4R37_RS22100	4427467..4428213	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
A4R37_RS22105	4428265..4428816	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
A4R37_RS22110	4428828..4429085	-	258	WP_001054379.1	YjhX family toxin	Toxin
A4R37_RS22115	4429462..4429761	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
A4R37_RS22120	4429839..4430570	+	732	Protein_4191	DNA helicase	-
A4R37_RS22125	4430600..4432213	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
A4R37_RS22130	4432244..4432594	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A4R37_RS22135	4432591..4433016	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A4R37_RS22140	4433102..4433605	+	504	Protein_4195	DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T62259 WP_001054379.1 NZ_CP015076:c4429085-4428828 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T62259 NZ_CP015076:c4429085-4428828 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT62259 WP_000123957.1 NZ_CP015076:c4428816-4428265 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT62259 NZ_CP015076:c4428816-4428265 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030