Detailed information of TA system TA062258

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4396882..4397716
• Replicon: chromosome
• Accession: NZ_CP015076
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: A4R37_RS21960
• Protein ID: WP_000854770.1
• Coordinates: 4397339..4397716 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: A4R37_RS21955
• Protein ID: WP_001280950.1
• Coordinates: 4396882..4397250 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS21915	4392034..4393935	+	1902	Protein_4151	Ag43/Cah family autotransporter adhesin	-
A4R37_RS21920	4394006..4394164	+	159	WP_001323397.1	DUF905 family protein	-
A4R37_RS21925	4394319..4395137	+	819	WP_001234712.1	DUF945 domain-containing protein	-
A4R37_RS21935	4395479..4395943	+	465	WP_000855061.1	antirestriction protein	-
A4R37_RS21940	4395959..4396435	+	477	WP_001186779.1	RadC family protein	-
A4R37_RS21945	4396498..4396719	+	222	WP_001220314.1	DUF987 domain-containing protein	-
A4R37_RS21955	4396882..4397250	+	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4R37_RS21960	4397339..4397716	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
A4R37_RS21965	4397713..4398201	+	489	WP_000761690.1	hypothetical protein	-
A4R37_RS21970	4398218..4398394	+	177	WP_000839286.1	DUF957 domain-containing protein	-
A4R37_RS29625	4398494..4398652	+	159	WP_001467148.1	hypothetical protein	-
A4R37_RS29300	4399016..4399192	+	177	Protein_4162	helix-turn-helix domain-containing protein	-
A4R37_RS21980	4399965..4401605	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T62258 WP_000854770.1 NZ_CP015076:4397339-4397716 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T62258 NZ_CP015076:4397339-4397716 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT62258 WP_001280950.1 NZ_CP015076:4396882-4397250 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT62258 NZ_CP015076:4396882-4397250 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885