Detailed information of TA system TA062256

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3600906..3601741
• Replicon: chromosome
• Accession: NZ_CP015076
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A376WVB1
• Locus tag: A4R37_RS18105
• Protein ID: WP_001094448.1
• Coordinates: 3601364..3601741 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3T7EC32
• Locus tag: A4R37_RS18100
• Protein ID: WP_024175935.1
• Coordinates: 3600906..3601274 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS18075	3598331..3598564	+	234	WP_001117568.1	DUF905 family protein	-
A4R37_RS18080	3598655..3599473	+	819	WP_001234753.1	DUF945 domain-containing protein	-
A4R37_RS18085	3599565..3600050	+	486	WP_063131919.1	antirestriction protein	-
A4R37_RS18090	3600066..3600542	+	477	WP_063131920.1	RadC family protein	-
A4R37_RS18095	3600611..3600832	+	222	WP_000692346.1	DUF987 domain-containing protein	-
A4R37_RS18100	3600906..3601274	+	369	WP_024175935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4R37_RS18105	3601364..3601741	+	378	WP_001094448.1	TA system toxin CbtA family protein	Toxin
A4R37_RS18110	3601738..3602226	+	489	WP_000761714.1	hypothetical protein	-
A4R37_RS18115	3602238..3602459	+	222	WP_001296561.1	DUF957 domain-containing protein	-
A4R37_RS18120	3602515..3603360	+	846	WP_001576423.1	DUF4942 domain-containing protein	-
A4R37_RS18130	3603953..3604450	+	498	WP_000509815.1	hypothetical protein	-
A4R37_RS18135	3604628..3605551	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
A4R37_RS18140	3605555..3606373	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3593167..3604450	11283

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14221.22 Da
• Isoelectric Point: 8.2919

>T62256 WP_001094448.1 NZ_CP015076:3601364-3601741 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T62256 NZ_CP015076:3601364-3601741 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13598.40 Da
• Isoelectric Point: 6.6255

>AT62256 WP_024175935.1 NZ_CP015076:3600906-3601274 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT62256 NZ_CP015076:3600906-3601274 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A376WVB1

Antitoxin

• AlphaFold DB: A0A3T7EC32