Detailed information of TA system TA062251

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2762656..2763490
• Replicon: chromosome
• Accession: NZ_CP015076
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: A4R37_RS13985
• Protein ID: WP_001546109.1
• Coordinates: 2763113..2763490 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: A4R37_RS13980
• Protein ID: WP_001546108.1
• Coordinates: 2762656..2763036 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS13945	2758936..2759391	+	456	WP_001545736.1	hypothetical protein	-
A4R37_RS13950	2759470..2759703	+	234	WP_001119729.1	DUF905 family protein	-
A4R37_RS13955	2759803..2760621	+	819	WP_001234620.1	DUF945 domain-containing protein	-
A4R37_RS13960	2760676..2761161	+	486	WP_000849588.1	antirestriction protein	-
A4R37_RS13965	2761177..2761653	+	477	WP_001424026.1	RadC family protein	-
A4R37_RS13970	2761722..2761943	+	222	WP_000692345.1	DUF987 domain-containing protein	-
A4R37_RS13975	2761962..2762606	+	645	WP_000086755.1	hypothetical protein	-
A4R37_RS13980	2762656..2763036	+	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4R37_RS13985	2763113..2763490	+	378	WP_001546109.1	TA system toxin CbtA family protein	Toxin
A4R37_RS13990	2763487..2763974	+	488	Protein_2664	hypothetical protein	-
A4R37_RS13995	2763991..2764188	+	198	WP_000839260.1	DUF957 domain-containing protein	-
A4R37_RS14000	2764273..2765118	+	846	WP_001280441.1	DUF4942 domain-containing protein	-
A4R37_RS14005	2765187..2765582	+	396	WP_000208383.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
A4R37_RS14010	2765575..2766509	+	935	Protein_2668	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
A4R37_RS29610	2766941..2767096	+	156	WP_000729639.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.00 Da
• Isoelectric Point: 8.5190

>T62251 WP_001546109.1 NZ_CP015076:2763113-2763490 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYKMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T62251 NZ_CP015076:2763113-2763490 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT62251 WP_001546108.1 NZ_CP015076:2762656-2763036 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT62251 NZ_CP015076:2762656-2763036 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8