Detailed information of TA system TA062233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4807573..4808393
• Replicon: chromosome
• Accession: NZ_CP015074
• Organism: Escherichia coli strain Ecol_745

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: A4R38_RS23830
• Protein ID: WP_001054379.1
• Coordinates: 4807573..4807830 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: A4R38_RS23835
• Protein ID: WP_000123957.1
• Coordinates: 4807842..4808393 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R38_RS23800	4803053..4803556	-	504	Protein_4535	DNA helicase	-
A4R38_RS23805	4803642..4804067	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A4R38_RS23810	4804064..4804414	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A4R38_RS23815	4804445..4806058	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
A4R38_RS23820	4806088..4806819	-	732	Protein_4539	DNA helicase	-
A4R38_RS23825	4806897..4807196	+	300	WP_001332034.1	GNAT family N-acetyltransferase	-
A4R38_RS23830	4807573..4807830	+	258	WP_001054379.1	YjhX family toxin	Toxin
A4R38_RS23835	4807842..4808393	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
A4R38_RS23840	4808445..4809191	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
A4R38_RS23845	4809319..4809579	+	261	WP_000077645.1	hypothetical protein	-
A4R38_RS28155	4809617..4809733	+	117	Protein_4545	VOC family protein	-
A4R38_RS23850	4809978..4811099	+	1122	WP_000010833.1	M42 family metallopeptidase	-
A4R38_RS23855	4811096..4811374	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A4R38_RS23860	4811386..4812699	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T62233 WP_001054379.1 NZ_CP015074:4807573-4807830 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T62233 NZ_CP015074:4807573-4807830 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT62233 WP_000123957.1 NZ_CP015074:4807842-4808393 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT62233 NZ_CP015074:4807842-4808393 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030