Detailed information of TA system TA062230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4428064..4429753
• Replicon: chromosome
• Accession: NZ_CP015074
• Organism: Escherichia coli strain Ecol_745

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: A4R38_RS21965
• Protein ID: WP_000365807.1
• Coordinates: 4428064..4428984 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: A4R38_RS21970
• Protein ID: WP_001295795.1
• Coordinates: 4428971..4429753 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R38_RS21935	4423537..4424247	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
A4R38_RS21940	4424554..4424883	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
A4R38_RS26635	4424895..4425058	-	164	Protein_4180	tail fiber assembly protein	-
A4R38_RS21945	4425132..4425746	-	615	WP_001019923.1	YagU family protein	-
A4R38_RS21950	4425961..4426146	-	186	WP_000146240.1	hypothetical protein	-
A4R38_RS28080	4426195..4426353	+	159	Protein_4183	integrase	-
A4R38_RS21955	4426580..4426915	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
A4R38_RS21960	4427123..4427899	+	777	WP_000171155.1	site-specific integrase	-
A4R38_RS21965	4428064..4428984	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A4R38_RS21970	4428971..4429753	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A4R38_RS21975	4430037..4433135	+	3099	WP_000673070.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4387851..4436003	48152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T62230 WP_000365807.1 NZ_CP015074:c4428984-4428064 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T62230 NZ_CP015074:c4428984-4428064 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT62230 WP_001295795.1 NZ_CP015074:c4429753-4428971 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT62230 NZ_CP015074:c4429753-4428971 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A061KTE0