Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4244775..4245393 Replicon chromosome
Accession NZ_CP015074
Organism Escherichia coli strain Ecol_745

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A4R38_RS21110 Protein ID WP_001291435.1
Coordinates 4245175..4245393 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A4R38_RS21105 Protein ID WP_000344800.1
Coordinates 4244775..4245149 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R38_RS21095 4239864..4241057 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A4R38_RS21100 4241080..4244229 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
A4R38_RS21105 4244775..4245149 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A4R38_RS21110 4245175..4245393 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A4R38_RS21115 4245566..4246117 + 552 WP_063131876.1 maltose O-acetyltransferase -
A4R38_RS21120 4246233..4246703 + 471 WP_000136192.1 YlaC family protein -
A4R38_RS21125 4246867..4248417 + 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A4R38_RS21130 4248459..4248812 - 354 WP_000878147.1 DUF1428 family protein -
A4R38_RS21140 4249191..4249502 + 312 WP_000409908.1 MGMT family protein -
A4R38_RS21145 4249533..4250105 - 573 WP_000779841.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62229 WP_001291435.1 NZ_CP015074:4245175-4245393 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62229 NZ_CP015074:4245175-4245393 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT62229 WP_000344800.1 NZ_CP015074:4244775-4245149 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT62229 NZ_CP015074:4244775-4245149 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References