62227

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3577770..3578241 Replicon chromosome
Accession NZ_CP015074
Organism Escherichia coli strain Ecol_745

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A4R38_RS17815 Protein ID WP_001303511.1
Coordinates 3577963..3578241 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A4R38_RS17810 Protein ID WP_001302048.1
Coordinates 3577770..3577961 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R38_RS17765 3573019..3573237 - 219 WP_001610644.1 DUF4014 family protein -
A4R38_RS17770 3573270..3573482 - 213 WP_000072553.1 hypothetical protein -
A4R38_RS17775 3573588..3574010 - 423 Protein_3386 DUF977 family protein -
A4R38_RS17780 3574026..3574796 - 771 WP_000450998.1 DUF1627 domain-containing protein -
A4R38_RS17785 3574818..3575564 - 747 WP_000788950.1 ATP-binding protein -
A4R38_RS17790 3575571..3576533 - 963 WP_000095673.1 helix-turn-helix domain-containing protein -
A4R38_RS17795 3576556..3576981 - 426 WP_000693943.1 Rha family transcriptional regulator -
A4R38_RS17800 3576978..3577280 - 303 WP_001556930.1 transcriptional regulator -
A4R38_RS27295 3577378..3577749 + 372 WP_001169687.1 hypothetical protein -
A4R38_RS17810 3577770..3577961 + 192 WP_001302048.1 hypothetical protein Antitoxin
A4R38_RS17815 3577963..3578241 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4R38_RS17820 3578532..3578684 + 153 WP_000380313.1 DUF1391 family protein -
A4R38_RS17825 3578696..3579034 + 339 WP_000394541.1 hypothetical protein -
A4R38_RS17830 3579023..3579289 - 267 WP_012601410.1 hypothetical protein -
A4R38_RS17840 3579793..3579981 + 189 WP_000449175.1 cell division inhibitor -
A4R38_RS17845 3579978..3580166 + 189 WP_000199475.1 DUF1482 family protein -
A4R38_RS17850 3580259..3582697 + 2439 WP_000102132.1 exonuclease -
A4R38_RS17855 3582759..3583028 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3560374..3587423 27049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T62227 WP_001303511.1 NZ_CP015074:3577963-3578241 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T62227 NZ_CP015074:3577963-3578241 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT62227 WP_001302048.1 NZ_CP015074:3577770-3577961 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT62227 NZ_CP015074:3577770-3577961 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References