Detailed information of TA system TA062226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3078889..3079415
• Replicon: chromosome
• Accession: NZ_CP015074
• Organism: Escherichia coli strain Ecol_745

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: A4R38_RS15100
• Protein ID: WP_000323025.1
• Coordinates: 3079128..3079415 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: A4R38_RS15095
• Protein ID: WP_000534858.1
• Coordinates: 3078889..3079128 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R38_RS15075	3074151..3074765	+	615	WP_001265627.1	hypothetical protein	-
A4R38_RS15080	3074762..3075790	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
A4R38_RS15085	3076274..3077587	+	1314	WP_000589005.1	ISNCY family transposase	-
A4R38_RS15090	3078024..3078356	-	333	WP_001301033.1	protein FlxA	-
A4R38_RS26220	3078559..3078864	-	306	WP_001326990.1	hypothetical protein	-
A4R38_RS15095	3078889..3079128	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
A4R38_RS15100	3079128..3079415	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
A4R38_RS15105	3079487..3079642	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
A4R38_RS15110	3079859..3080110	+	252	WP_000980994.1	hypothetical protein	-
A4R38_RS15115	3080177..3080455	+	279	WP_012304870.1	hypothetical protein	-
A4R38_RS15120	3080457..3081506	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
A4R38_RS15125	3081520..3082272	+	753	WP_001047135.1	antitermination protein	-
A4R38_RS27720	3082550..3082639	-	90	WP_120795389.1	hypothetical protein	-
A4R38_RS15130	3082694..3082906	-	213	WP_000087756.1	cold shock-like protein CspF	-
A4R38_RS15135	3083207..3083422	+	216	WP_000066484.1	cold shock-like protein CspB	-
A4R38_RS28255	3083786..3083956	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
A4R38_RS15140	3084176..3084391	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3053600..3115022	61422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T62226 WP_000323025.1 NZ_CP015074:3079128-3079415 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T62226 NZ_CP015074:3079128-3079415 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT62226 WP_000534858.1 NZ_CP015074:3078889-3079128 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT62226 NZ_CP015074:3078889-3079128 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8