62217

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2591924..2592755 Replicon chromosome
Accession NZ_CP015074
Organism Escherichia coli strain Ecol_745

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag A4R38_RS12525 Protein ID WP_000854814.1
Coordinates 2591924..2592298 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A641DSP2
Locus tag A4R38_RS12530 Protein ID WP_001546021.1
Coordinates 2592387..2592755 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R38_RS12490 2587919..2588248 + 330 WP_000450409.1 DUF496 family protein -
A4R38_RS12495 2588349..2588531 - 183 WP_001016348.1 hypothetical protein -
A4R38_RS12500 2588585..2588731 + 147 Protein_2363 transposase domain-containing protein -
A4R38_RS12505 2588942..2590483 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
A4R38_RS12510 2590498..2591244 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
A4R38_RS27655 2591607..2591720 - 114 WP_001161660.1 DUF957 domain-containing protein -
A4R38_RS12520 2591733..2591927 - 195 WP_000988601.1 hypothetical protein -
A4R38_RS12525 2591924..2592298 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
A4R38_RS12530 2592387..2592755 - 369 WP_001546021.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
A4R38_RS12535 2592835..2593056 - 222 WP_000692323.1 DUF987 domain-containing protein -
A4R38_RS12540 2593119..2593595 - 477 WP_001186773.1 RadC family protein -
A4R38_RS12545 2593611..2594084 - 474 WP_001385393.1 antirestriction protein -
A4R38_RS12550 2594347..2595168 - 822 WP_001234710.1 DUF945 domain-containing protein -
A4R38_RS12555 2595389..2595799 - 411 WP_000846704.1 hypothetical protein -
A4R38_RS12560 2595815..2596492 - 678 WP_001362823.1 hypothetical protein -
A4R38_RS12565 2596628..2597698 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T62217 WP_000854814.1 NZ_CP015074:c2592298-2591924 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T62217 NZ_CP015074:c2592298-2591924 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13607.47 Da        Isoelectric Point: 6.3139

>AT62217 WP_001546021.1 NZ_CP015074:c2592755-2592387 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT62217 NZ_CP015074:c2592755-2592387 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A641DSP2

References