Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 6258..6859 Replicon plasmid pEC743_1
Accession NZ_CP015070
Organism Escherichia coli strain Ecol_743

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag A4R39_RS23885 Protein ID WP_001216034.1
Coordinates 6479..6859 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag A4R39_RS23880 Protein ID WP_001190712.1
Coordinates 6258..6479 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R39_RS23870 3239..4522 - 1284 WP_001617890.1 restriction endonuclease subunit S -
A4R39_RS23875 4519..6075 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
A4R39_RS23880 6258..6479 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A4R39_RS23885 6479..6859 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
A4R39_RS23890 6864..7043 + 180 WP_001513661.1 hypothetical protein -
A4R39_RS23895 7071..7430 + 360 WP_001513660.1 hypothetical protein -
A4R39_RS23900 7354..7767 + 414 Protein_7 DDE-type integrase/transposase/recombinase -
A4R39_RS23905 7717..8034 - 318 WP_001513659.1 hypothetical protein -
A4R39_RS23910 8262..9242 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
A4R39_RS23915 9486..10889 + 1404 WP_001373486.1 S-methylmethionine permease -
A4R39_RS23920 10876..11808 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..111851 111851


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T62195 WP_001216034.1 NZ_CP015070:6479-6859 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T62195 NZ_CP015070:6479-6859 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT62195 WP_001190712.1 NZ_CP015070:6258-6479 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT62195 NZ_CP015070:6258-6479 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References