Detailed information of TA system TA062189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3574811..3575631
• Replicon: chromosome
• Accession: NZ_CP015069
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: A4R39_RS17500
• Protein ID: WP_001054379.1
• Coordinates: 3575374..3575631 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: A4R39_RS17495
• Protein ID: WP_000123957.1
• Coordinates: 3574811..3575362 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS17470	3570505..3571818	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
A4R39_RS17475	3571830..3572108	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A4R39_RS17480	3572105..3573226	-	1122	WP_000010833.1	M42 family metallopeptidase	-
A4R39_RS28930	3573471..3573587	-	117	Protein_3310	VOC family protein	-
A4R39_RS17485	3573625..3573885	-	261	WP_000077645.1	hypothetical protein	-
A4R39_RS17490	3574013..3574759	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
A4R39_RS17495	3574811..3575362	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
A4R39_RS17500	3575374..3575631	-	258	WP_001054379.1	YjhX family toxin	Toxin
A4R39_RS17505	3576008..3576307	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
A4R39_RS17510	3576385..3577116	+	732	Protein_3316	DNA helicase	-
A4R39_RS17515	3577146..3578759	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
A4R39_RS17520	3578790..3579140	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A4R39_RS17525	3579137..3579562	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A4R39_RS17530	3579648..3580151	+	504	Protein_3320	DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T62189 WP_001054379.1 NZ_CP015069:c3575631-3575374 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T62189 NZ_CP015069:c3575631-3575374 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT62189 WP_000123957.1 NZ_CP015069:c3575362-3574811 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT62189 NZ_CP015069:c3575362-3574811 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030