Detailed information of TA system TA062188

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3543428..3544262
• Replicon: chromosome
• Accession: NZ_CP015069
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: A4R39_RS17350
• Protein ID: WP_000854770.1
• Coordinates: 3543885..3544262 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: A4R39_RS17345
• Protein ID: WP_001280950.1
• Coordinates: 3543428..3543796 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS17305	3538580..3540481	+	1902	Protein_3277	Ag43/Cah family autotransporter adhesin	-
A4R39_RS17310	3540552..3540710	+	159	WP_001323397.1	DUF905 family protein	-
A4R39_RS17315	3540865..3541683	+	819	WP_001234712.1	DUF945 domain-containing protein	-
A4R39_RS17325	3542025..3542489	+	465	WP_000855061.1	antirestriction protein	-
A4R39_RS17330	3542505..3542981	+	477	WP_001186779.1	RadC family protein	-
A4R39_RS17335	3543044..3543265	+	222	WP_001220314.1	DUF987 domain-containing protein	-
A4R39_RS17345	3543428..3543796	+	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4R39_RS17350	3543885..3544262	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
A4R39_RS17355	3544259..3544747	+	489	WP_000761690.1	hypothetical protein	-
A4R39_RS17360	3544764..3544940	+	177	WP_000839286.1	DUF957 domain-containing protein	-
A4R39_RS28565	3545562..3545738	+	177	Protein_3287	helix-turn-helix domain-containing protein	-
A4R39_RS17370	3546511..3548151	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3495430..3555908	60478

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T62188 WP_000854770.1 NZ_CP015069:3543885-3544262 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T62188 NZ_CP015069:3543885-3544262 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT62188 WP_001280950.1 NZ_CP015069:3543428-3543796 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT62188 NZ_CP015069:3543428-3543796 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885