Detailed information of TA system TA062181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2504202..2505002
• Replicon: chromosome
• Accession: NZ_CP015069
• Organism: Escherichia coli strain Ecol_743

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: A4R39_RS12370
• Protein ID: WP_000342452.1
• Coordinates: 2504202..2504729 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: A4R39_RS12375
• Protein ID: WP_001277107.1
• Coordinates: 2504736..2505002 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R39_RS12345	2499278..2500045	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
A4R39_RS12350	2500042..2501319	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
A4R39_RS12355	2501316..2502242	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
A4R39_RS12360	2502290..2503399	-	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
A4R39_RS12365	2503822..2504205	+	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
A4R39_RS12370	2504202..2504729	-	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
A4R39_RS12375	2504736..2505002	-	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
A4R39_RS12380	2505151..2506254	-	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
A4R39_RS12385	2506525..2507379	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
A4R39_RS12390	2507624..2508682	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
A4R39_RS12395	2508675..2509343	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T62181 WP_000342452.1 NZ_CP015069:c2504729-2504202 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T62181 NZ_CP015069:c2504729-2504202 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT62181 WP_001277107.1 NZ_CP015069:c2505002-2504736 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT62181 NZ_CP015069:c2505002-2504736 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57