Detailed information of TA system TA062158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): slvAT/RES(toxin)
• Location: 58626..59185
• Replicon: plasmid pMc1284
• Accession: NZ_CP015065
• Organism: Mesorhizobium ciceri biovar biserrulae strain WSM1284

Toxin (Protein)

• Gene name: slvT
• Uniprot ID: -
• Locus tag: A4R29_RS34350
• Protein ID: WP_145923805.1
• Coordinates: 59009..59185 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: slvA
• Uniprot ID: E8TNW3
• Locus tag: A4R29_RS30170
• Protein ID: WP_013525337.1
• Coordinates: 58626..59012 (+)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R29_RS30165	57431..58361	+	931	Protein_45	type I restriction-modification system subunit M	-
A4R29_RS30170	58626..59012	+	387	WP_013525337.1	hypothetical protein	Antitoxin
A4R29_RS34350	59009..59185	+	177	WP_145923805.1	RES domain-containing protein	Toxin
A4R29_RS30175	59206..62181	-	2976	Protein_48	Tn3 family transposase	-
A4R29_RS30180	62338..62808	+	471	WP_082827602.1	ABC transporter permease subunit	-
A4R29_RS30185	62814..63845	+	1032	WP_063170518.1	histone deacetylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..553641	553641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6376.22 Da
• Isoelectric Point: 6.9738

>T62158 WP_145923805.1 NZ_CP015065:59009-59185 [Mesorhizobium ciceri biovar biserrulae]
VKVTRVGPDEIFHRYLTPKWAFLPTSGGGAAIDGGRFNRPGVEAVYLGSEQEQCEIVR

Nucleotide

• Length: 177 bp

>T62158 NZ_CP015065:59009-59185 [Mesorhizobium ciceri biovar biserrulae]
GTGAAGGTCACCCGCGTCGGGCCGGACGAGATCTTCCATCGCTATCTGACACCCAAATGGGCCTTCCTGCCCACCAGTGG
TGGGGGCGCGGCGATCGATGGCGGGCGCTTCAATCGGCCCGGCGTCGAAGCCGTTTACCTGGGGTCGGAACAAGAGCAGT
GCGAAATCGTACGCTAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14030.97 Da
• Isoelectric Point: 9.8613

>AT62158 WP_013525337.1 NZ_CP015065:58626-59012 [Mesorhizobium ciceri biovar biserrulae]
MTHAQLAQSKVVSGFVDSLQEPRTPYISPKRLSQALGVKVANLAQLTGVHRNTLRNPSSERLQGRMREMVKVISAATELT
GDVDKAIYWYRNEPIADYGHRTAAELVAGGQVEAVLAFIRDLENGARG

Nucleotide

• Length: 387 bp

>AT62158 NZ_CP015065:58626-59012 [Mesorhizobium ciceri biovar biserrulae]
ATGACCCACGCACAGCTCGCACAATCGAAAGTGGTCTCAGGTTTTGTCGACAGCCTGCAGGAGCCGCGCACGCCTTATAT
CTCGCCGAAGCGCCTGTCGCAGGCGCTGGGCGTGAAGGTGGCCAACTTGGCGCAGTTGACCGGCGTCCACCGCAACACGC
TCCGTAACCCGTCGTCGGAGCGACTGCAGGGTCGGATGCGCGAGATGGTGAAGGTGATTTCGGCCGCAACGGAACTCACC
GGCGACGTCGACAAGGCGATCTACTGGTATCGCAATGAGCCGATCGCCGACTATGGCCATCGCACGGCGGCCGAACTCGT
CGCGGGCGGCCAGGTCGAAGCAGTGCTGGCCTTCATCCGGGATCTCGAGAACGGGGCGCGCGGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A436N5N0