Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4195686..4196467 Replicon chromosome
Accession NZ_CP015064
Organism Mesorhizobium ciceri biovar biserrulae strain WSM1284

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A3S2GFV8
Locus tag A4R29_RS19720 Protein ID WP_063168651.1
Coordinates 4195973..4196467 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID E8T9Q9
Locus tag A4R29_RS19715 Protein ID WP_013530564.1
Coordinates 4195686..4195976 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R29_RS19685 4191573..4192415 + 843 WP_063172659.1 helix-turn-helix transcriptional regulator -
A4R29_RS19690 4192453..4192899 - 447 WP_063172660.1 SRPBCC domain-containing protein -
A4R29_RS19695 4193058..4193810 + 753 WP_063172661.1 rRNA pseudouridine synthase -
A4R29_RS19700 4193931..4194332 + 402 WP_063173748.1 VOC family protein -
A4R29_RS19705 4194363..4194944 - 582 WP_063172662.1 histidine phosphatase family protein -
A4R29_RS19710 4195152..4195601 + 450 WP_027160859.1 universal stress protein -
A4R29_RS19715 4195686..4195976 + 291 WP_013530564.1 DUF1778 domain-containing protein Antitoxin
A4R29_RS19720 4195973..4196467 + 495 WP_063168651.1 GNAT family N-acetyltransferase Toxin
A4R29_RS19725 4196503..4197252 - 750 WP_063172663.1 sulfite exporter TauE/SafE family protein -
A4R29_RS19730 4197274..4197480 - 207 WP_063172664.1 hypothetical protein -
A4R29_RS19735 4197618..4198490 + 873 WP_013530561.1 LysR family transcriptional regulator -
A4R29_RS19740 4198622..4199122 + 501 WP_024501775.1 Lrp/AsnC family transcriptional regulator -
A4R29_RS19745 4199119..4200282 + 1164 WP_013530559.1 saccharopine dehydrogenase family protein -
A4R29_RS19750 4200393..4201199 + 807 WP_013530558.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(43-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17511.18 Da        Isoelectric Point: 8.8832

>T62157 WP_063168651.1 NZ_CP015064:4195973-4196467 [Mesorhizobium ciceri biovar biserrulae]
VTLSVPVPLADHHELGEFSSGITALDDWLKKRARTNQAGGASRTYVTCKGNRVIGYYAVASGAVKMVEASSRFRRNMPDP
IPVAVLGRLAVDQAYHGRGIGRALVRDAGLRILQAAEILGIKGIVVHAISDDARAFYITIGFEPSPVEPLTLMVTLADLS
ESIG

Download         Length: 495 bp

>T62157 NZ_CP015064:4195973-4196467 [Mesorhizobium ciceri biovar biserrulae]
GTGACGCTGTCGGTCCCGGTTCCACTCGCGGATCATCACGAACTCGGGGAATTTTCGTCGGGCATTACAGCGCTCGACGA
TTGGTTGAAAAAACGTGCTCGCACCAATCAAGCGGGCGGTGCATCCCGGACCTATGTGACTTGCAAAGGCAACCGTGTGA
TTGGCTACTATGCCGTTGCCAGCGGCGCCGTGAAAATGGTCGAAGCTTCCAGCCGTTTCCGGCGCAACATGCCCGATCCG
ATCCCCGTCGCAGTGCTAGGCAGGTTGGCCGTAGACCAAGCCTATCACGGTCGGGGCATCGGTCGGGCGCTGGTAAGGGA
TGCCGGTCTACGAATTCTCCAGGCTGCTGAAATACTGGGCATCAAAGGCATAGTGGTGCATGCGATTTCCGACGATGCCC
GAGCTTTCTACATCACCATCGGGTTCGAGCCCTCGCCGGTCGAGCCGTTGACACTCATGGTCACGCTCGCTGATCTCAGC
GAAAGCATTGGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10842.51 Da        Isoelectric Point: 9.9898

>AT62157 WP_013530564.1 NZ_CP015064:4195686-4195976 [Mesorhizobium ciceri biovar biserrulae]
MPTSHHPVPKSVPLNIRVKPEVRNLIDRAAELLGKNRTDFMLEASQRAAEEALLNRLVFTVEPDVYAAFLARLDAPPQPN
DRLRRTMTTKAPWEAA

Download         Length: 291 bp

>AT62157 NZ_CP015064:4195686-4195976 [Mesorhizobium ciceri biovar biserrulae]
ATGCCAACGTCACATCATCCGGTGCCGAAGAGCGTTCCGCTCAACATCCGCGTCAAGCCGGAAGTTCGCAATCTGATCGA
CCGCGCAGCCGAATTGCTCGGTAAGAATCGCACCGACTTCATGCTTGAAGCCTCGCAACGCGCCGCTGAGGAAGCGTTGC
TCAACCGTCTCGTGTTTACCGTCGAACCGGATGTCTACGCTGCGTTTCTGGCACGCCTCGACGCGCCGCCGCAGCCCAAC
GACCGATTGCGCCGCACGATGACGACAAAGGCTCCGTGGGAAGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S2GFV8

Antitoxin

Source ID Structure
AlphaFold DB A0A3S2IG73

References