Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2808432..2809213 Replicon chromosome
Accession NZ_CP015062
Organism Mesorhizobium ciceri strain CC1192

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A3S2GFV8
Locus tag A4R28_RS13885 Protein ID WP_063168651.1
Coordinates 2808719..2809213 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID E8T9Q9
Locus tag A4R28_RS13880 Protein ID WP_013530564.1
Coordinates 2808432..2808722 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4R28_RS13850 2804312..2805154 + 843 WP_063168648.1 helix-turn-helix transcriptional regulator -
A4R28_RS13855 2805196..2805642 - 447 WP_063168649.1 SRPBCC domain-containing protein -
A4R28_RS13860 2805801..2806553 + 753 WP_063170295.1 rRNA pseudouridine synthase -
A4R28_RS13865 2806674..2807075 + 402 WP_013530567.1 VOC family protein -
A4R28_RS13870 2807106..2807687 - 582 WP_063168650.1 histidine phosphatase family protein -
A4R28_RS13875 2807896..2808345 + 450 WP_027160859.1 universal stress protein -
A4R28_RS13880 2808432..2808722 + 291 WP_013530564.1 DUF1778 domain-containing protein Antitoxin
A4R28_RS13885 2808719..2809213 + 495 WP_063168651.1 GNAT family N-acetyltransferase Toxin
A4R28_RS13890 2809249..2809998 - 750 WP_063168652.1 sulfite exporter TauE/SafE family protein -
A4R28_RS13895 2810020..2810226 - 207 WP_063168653.1 hypothetical protein -
A4R28_RS13900 2810364..2811236 + 873 WP_063170296.1 LysR family transcriptional regulator -
A4R28_RS13905 2811368..2811868 + 501 WP_024501775.1 Lrp/AsnC family transcriptional regulator -
A4R28_RS13910 2811865..2813028 + 1164 WP_063168654.1 saccharopine dehydrogenase family protein -
A4R28_RS13915 2813139..2813945 + 807 WP_013530558.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(43-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17511.18 Da        Isoelectric Point: 8.8832

>T62154 WP_063168651.1 NZ_CP015062:2808719-2809213 [Mesorhizobium ciceri]
VTLSVPVPLADHHELGEFSSGITALDDWLKKRARTNQAGGASRTYVTCKGNRVIGYYAVASGAVKMVEASSRFRRNMPDP
IPVAVLGRLAVDQAYHGRGIGRALVRDAGLRILQAAEILGIKGIVVHAISDDARAFYITIGFEPSPVEPLTLMVTLADLS
ESIG

Download         Length: 495 bp

>T62154 NZ_CP015062:2808719-2809213 [Mesorhizobium ciceri]
GTGACGCTGTCGGTCCCGGTTCCACTCGCGGATCATCACGAACTCGGGGAATTTTCGTCGGGCATTACAGCGCTCGACGA
TTGGTTGAAAAAACGTGCTCGCACCAATCAAGCGGGCGGTGCATCCCGGACCTATGTGACTTGCAAAGGCAACCGTGTGA
TTGGCTACTATGCCGTTGCCAGCGGCGCCGTGAAAATGGTCGAAGCTTCCAGCCGTTTCCGGCGCAACATGCCCGATCCG
ATCCCCGTCGCAGTGCTAGGCAGGTTGGCCGTAGACCAAGCCTATCACGGTCGGGGCATCGGTCGGGCGCTGGTAAGGGA
TGCCGGTCTACGAATTCTCCAGGCTGCTGAAATACTGGGCATCAAAGGCATAGTGGTGCATGCGATTTCCGACGATGCCC
GAGCTTTCTACATCACCATCGGGTTCGAGCCCTCGCCGGTCGAGCCGTTGACACTCATGGTCACGCTCGCTGATCTCAGC
GAAAGCATTGGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10842.51 Da        Isoelectric Point: 9.9898

>AT62154 WP_013530564.1 NZ_CP015062:2808432-2808722 [Mesorhizobium ciceri]
MPTSHHPVPKSVPLNIRVKPEVRNLIDRAAELLGKNRTDFMLEASQRAAEEALLNRLVFTVEPDVYAAFLARLDAPPQPN
DRLRRTMTTKAPWEAA

Download         Length: 291 bp

>AT62154 NZ_CP015062:2808432-2808722 [Mesorhizobium ciceri]
ATGCCAACGTCACATCATCCGGTGCCGAAGAGCGTTCCGCTCAACATCCGCGTCAAGCCGGAAGTTCGCAATCTGATCGA
CCGCGCAGCCGAATTGCTCGGTAAGAATCGCACCGACTTCATGCTTGAAGCCTCGCAACGCGCCGCTGAGGAAGCGTTGC
TCAACCGTCTCGTGTTTACCGTCGAACCGGATGTCTACGCTGCGTTTCTGGCACGCCTCGACGCGCCGCCGCAGCCCAAC
GACCGATTGCGCCGCACGATGACGACAAAGGCTCCGTGGGAAGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S2GFV8

Antitoxin

Source ID Structure
AlphaFold DB A0A3S2IG73

References