Detailed information of TA system TA062150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 22615..23222
• Replicon: plasmid pRGUI02
• Accession: NZ_CP015041
• Organism: Rhodovulum sp. P5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RGUI_RS20350
• Protein ID: WP_081536303.1
• Coordinates: 22971..23222 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RGUI_RS20345
• Protein ID: WP_081536302.1
• Coordinates: 22615..22959 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RGUI_RS20335	18612..20804	-	2193	WP_081536301.1	efflux RND transporter periplasmic adaptor subunit	-
RGUI_RS21615	21121..21441	+	321	WP_156883121.1	hypothetical protein	-
RGUI_RS20340	21738..22496	-	759	WP_081536341.1	hypothetical protein	-
RGUI_RS20345	22615..22959	-	345	WP_081536302.1	helix-turn-helix transcriptional regulator	Antitoxin
RGUI_RS20350	22971..23222	-	252	WP_081536303.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RGUI_RS20355	23542..24514	-	973	Protein_21	IS5 family transposase	-
RGUI_RS20360	24670..25071	-	402	WP_172841229.1	PIN domain-containing protein	-
RGUI_RS20365	25064..25297	-	234	WP_081536305.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
RGUI_RS20370	25606..26808	-	1203	Protein_24	Tn3 family transposase	-
RGUI_RS21810	26731..27105	+	375	WP_172841227.1	hypothetical protein	-
RGUI_RS20375	27209..27616	-	408	WP_081536306.1	hypothetical protein	-
RGUI_RS20380	27622..28014	+	393	WP_081536307.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67286	67286
	-	inside	IScluster/Tn	-	-	23939..29403	5464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (58-112)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9242.66 Da
• Isoelectric Point: 11.1529

>T62150 WP_081536303.1 NZ_CP015041:c23222-22971 [Rhodovulum sp. P5]
MKQISYTKAAIRALRRMPANTATLIRSKIEAYARDPASQSNNVRSLKGREGIRLRVGDWRVIMDDQGNVLAVLDIGPRGG
VYD

Nucleotide

• Length: 252 bp

>T62150 NZ_CP015041:c23222-22971 [Rhodovulum sp. P5]
ATGAAGCAGATCAGCTACACGAAGGCCGCGATCCGGGCTTTGCGCCGGATGCCCGCCAACACGGCCACGCTGATCCGATC
CAAGATCGAGGCCTATGCGAGGGACCCGGCCTCACAGAGCAACAACGTGAGGTCCCTCAAGGGGCGAGAGGGGATCCGGC
TGCGGGTCGGGGACTGGCGCGTGATCATGGATGACCAGGGCAATGTCTTGGCGGTTCTCGATATTGGCCCACGTGGTGGC
GTTTATGACTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12401.93 Da
• Isoelectric Point: 4.3246

>AT62150 WP_081536302.1 NZ_CP015041:c22959-22615 [Rhodovulum sp. P5]
MNEMVTIPREEYDRLRAAAEDLADLQNYGRAKGALAEGADELIPADYANRLLDGENPLRVYRDLRGLTQAMLAEKAGVNR
VTVAEIETGRKQGSVASLRALAGALDVTLDDLTE

Nucleotide

• Length: 345 bp

>AT62150 NZ_CP015041:c22959-22615 [Rhodovulum sp. P5]
ATGAACGAGATGGTGACGATCCCGCGCGAGGAATATGACCGCCTGCGGGCCGCGGCCGAGGACCTCGCCGATCTCCAGAA
CTACGGTCGGGCAAAGGGGGCCTTGGCCGAAGGGGCGGATGAACTGATTCCCGCTGACTACGCGAACCGGCTGCTGGACG
GTGAAAATCCTCTCCGCGTTTACCGGGATCTGCGTGGCCTGACCCAAGCCATGCTAGCCGAGAAAGCAGGGGTCAATCGC
GTCACCGTGGCCGAGATCGAAACGGGCCGGAAACAGGGTTCGGTCGCCAGCTTGCGTGCCCTCGCGGGCGCCCTCGACGT
CACGCTGGACGATCTGACGGAGTAG