Detailed information of TA system TA062146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 5125330..5125862
• Replicon: chromosome
• Accession: NZ_CP015036
• Organism: Burkholderia cenocepacia strain 895

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A3203_RS23045
• Protein ID: WP_062910979.1
• Coordinates: 5125539..5125862 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: A3203_RS41025
• Protein ID: WP_012492929.1
• Coordinates: 5125330..5125542 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3203_RS23010	5120784..5122079	+	1296	WP_012492927.1	Tol-Pal system protein TolB	-
A3203_RS23015	5122097..5122609	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
A3203_RS23020	5122629..5123378	+	750	WP_006484965.1	tol-pal system protein YbgF	-
A3203_RS23030	5123806..5124126	-	321	WP_062910978.1	helix-turn-helix domain-containing protein	-
A3203_RS23035	5124267..5124662	+	396	WP_048986370.1	hypothetical protein	-
A3203_RS23040	5124730..5125182	+	453	WP_006484947.1	hypothetical protein	-
A3203_RS41025	5125330..5125542	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
A3203_RS23045	5125539..5125862	+	324	WP_062910979.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A3203_RS23050	5126182..5126706	+	525	WP_062910980.1	hypothetical protein	-
A3203_RS41030	5126708..5127742	+	1035	WP_080466919.1	relaxase/mobilization nuclease domain-containing protein	-
A3203_RS23060	5127865..5128308	-	444	WP_011544671.1	YiaA/YiaB family protein	-
A3203_RS23065	5128412..5129359	-	948	WP_062910982.1	AraC family transcriptional regulator	-
A3203_RS23070	5129489..5129818	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
A3203_RS23075	5130019..5130852	+	834	WP_034200961.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11686.78 Da
• Isoelectric Point: 8.9366

>T62146 WP_062910979.1 NZ_CP015036:5125539-5125862 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPFIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T62146 NZ_CP015036:5125539-5125862 [Burkholderia cenocepacia]
ATGAATCGCGGGAGCATTGTCACAGTCGCGCTCCAAGGTGACTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGCGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTCATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT62146 WP_012492929.1 NZ_CP015036:5125330-5125542 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT62146 NZ_CP015036:5125330-5125542 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATTCACCGCGAGAATCTTCGAGCGGCCGGGCTACGTCCAATTCAGATATG
GGTGCCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGTTACCGACCTCGGACACTCACAATGA

Structures

Antitoxin

• AlphaFold DB: B4EDC7