Detailed information of TA system TA062145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4705436..4706478
• Replicon: chromosome
• Accession: NZ_CP015036
• Organism: Burkholderia cenocepacia strain 895

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: A3203_RS20980
• Protein ID: WP_003153636.1
• Coordinates: 4705436..4706011 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: A3203_RS20985
• Protein ID: WP_003050245.1
• Coordinates: 4706008..4706478 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3203_RS20955	4701874..4702599	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
A3203_RS20960	4702638..4703540	-	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
A3203_RS20965	4703540..4704040	-	501	WP_003090159.1	hypothetical protein	-
A3203_RS20970	4704037..4704507	-	471	WP_003153638.1	hypothetical protein	-
A3203_RS20975	4704504..4705418	-	915	WP_003050256.1	AAA family ATPase	-
A3203_RS20980	4705436..4706011	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
A3203_RS20985	4706008..4706478	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
A3203_RS20990	4706682..4707065	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
A3203_RS20995	4707062..4707295	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
A3203_RS21000	4707312..4707671	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
A3203_RS21005	4707684..4708082	+	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
A3203_RS21010	4708079..4708771	+	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
A3203_RS21015	4708768..4709679	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
A3203_RS21020	4709669..4711087	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4650402..4754035	103633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T62145 WP_003153636.1 NZ_CP015036:c4706011-4705436 [Burkholderia cenocepacia]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T62145 NZ_CP015036:c4706011-4705436 [Burkholderia cenocepacia]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT62145 WP_003050245.1 NZ_CP015036:c4706478-4706008 [Burkholderia cenocepacia]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT62145 NZ_CP015036:c4706478-4706008 [Burkholderia cenocepacia]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68