Detailed information of TA system TA062144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3090444..3091090
• Replicon: chromosome
• Accession: NZ_CP015036
• Organism: Burkholderia cenocepacia strain 895

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A3203_RS13650
• Protein ID: WP_058902965.1
• Coordinates: 3090444..3090851 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4EGU8
• Locus tag: A3203_RS13655
• Protein ID: WP_006491146.1
• Coordinates: 3090848..3091090 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3203_RS13635	3086339..3086920	-	582	WP_058902967.1	hypothetical protein	-
A3203_RS13640	3086917..3087645	-	729	WP_058903588.1	chemotaxis protein	-
A3203_RS13645	3087668..3090043	-	2376	WP_062910522.1	MCP four helix bundle domain-containing protein	-
A3203_RS40330	3090101..3090328	+	228	WP_077021721.1	hypothetical protein	-
A3203_RS13650	3090444..3090851	-	408	WP_058902965.1	type II toxin-antitoxin system VapC family toxin	Toxin
A3203_RS13655	3090848..3091090	-	243	WP_006491146.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A3203_RS13660	3091349..3092032	+	684	WP_058902964.1	phospholipase	-
A3203_RS13665	3092104..3093609	-	1506	WP_006491141.1	amino acid permease	-
A3203_RS13670	3093688..3095094	-	1407	WP_006491136.1	aspartate ammonia-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14893.96 Da
• Isoelectric Point: 5.8982

>T62144 WP_058902965.1 NZ_CP015036:c3090851-3090444 [Burkholderia cenocepacia]
MKFLLDTNAVIAILKGEPAILARLHAWHPADFGIPAIVAHELYYGAYKSQRAAANVARVDALQFEVVSFDTEDAQHAGEI
RAHLIAAGTPIGPYDALIAGQARARQLVLVTHNVREFERVPRLQFEDWLAEPNAD

Nucleotide

• Length: 408 bp

>T62144 NZ_CP015036:c3090851-3090444 [Burkholderia cenocepacia]
ATGAAATTCCTGCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGGATTCCCGCGATCGTCGCGCACGAGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCTG
CGAACGTCGCGCGTGTCGACGCGTTGCAGTTCGAGGTCGTATCGTTCGACACCGAGGATGCACAGCATGCGGGTGAAATT
CGCGCGCACCTGATCGCGGCCGGCACGCCGATTGGTCCATACGACGCGTTGATCGCAGGGCAGGCGCGCGCACGACAACT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTCGCCGAACCGAACG
CAGACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.02 Da
• Isoelectric Point: 5.0197

>AT62144 WP_006491146.1 NZ_CP015036:c3091090-3090848 [Burkholderia cenocepacia]
MDTAKLFKHGGSQAVRLPKDFRFDTAEVRIRRHGAAVILEPMPQDWAWLAPLIGPVDADFEAAATTQPSAQERSGLDVFE

Nucleotide

• Length: 243 bp

>AT62144 NZ_CP015036:c3091090-3090848 [Burkholderia cenocepacia]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTACCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCAGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: B4EGU8