Detailed information of TA system TA062133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 126772..127304
• Replicon: chromosome
• Accession: NZ_CP015033
• Organism: Burkholderia cenocepacia strain 842

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1JWG6
• Locus tag: A2T82_RS01650
• Protein ID: WP_011544674.1
• Coordinates: 126981..127304 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A2T82_RS36380
• Protein ID: WP_011544675.1
• Coordinates: 126772..126984 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2T82_RS01615	122178..122690	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
A2T82_RS01620	122710..123459	+	750	WP_062884074.1	tol-pal system protein YbgF	-
A2T82_RS01630	123747..124997	-	1251	WP_034200813.1	IS256 family transposase	-
A2T82_RS01635	125259..125579	-	321	WP_196217732.1	helix-turn-helix domain-containing protein	-
A2T82_RS01640	125717..126112	+	396	WP_196217733.1	hypothetical protein	-
A2T82_RS01645	126180..126659	+	480	WP_011694172.1	hypothetical protein	-
A2T82_RS36380	126772..126984	+	213	WP_011544675.1	antitoxin MazE family protein	Antitoxin
A2T82_RS01650	126981..127304	+	324	WP_011544674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A2T82_RS36385	127624..128148	+	525	WP_077178415.1	hypothetical protein	-
A2T82_RS36390	128150..129184	+	1035	WP_077178414.1	relaxase/mobilization nuclease domain-containing protein	-
A2T82_RS01660	129307..129750	-	444	WP_011544671.1	YiaA/YiaB family protein	-
A2T82_RS01665	129854..130801	-	948	WP_062884077.1	AraC family transcriptional regulator	-
A2T82_RS01670	130931..131260	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
A2T82_RS01675	131455..132288	+	834	WP_054927435.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	123747..124997	1250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11668.77 Da
• Isoelectric Point: 8.9366

>T62133 WP_011544674.1 NZ_CP015033:126981-127304 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T62133 NZ_CP015033:126981-127304 [Burkholderia cenocepacia]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTTGGGCTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8026.00 Da
• Isoelectric Point: 6.2095

>AT62133 WP_011544675.1 NZ_CP015033:126772-126984 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRRPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT62133 NZ_CP015033:126772-126984 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTGCCCGACGTCCGCCGGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B1JWG6